Lysophosphatidic Acid Enhances In Vivo Infiltration and Activation of Guinea Pig Eosinophils and Neutrophils via a Rho/Rho-Associated Protein Kinase-Mediated Pathway
スポンサーリンク
概要
- 論文の詳細を見る
Lysophosphatidic acid (LPA) has been shown to be a chemoattractant in in vitro studies. The present study was carried out to determine whether LPA enhances infiltration of inflammatory cells in in vivo studies with guinea pigs. LPA (1 – 10 μg/ml), when by guinea pigs for 5 min, substantially increased the numbers of eosinophils and neutrophils in the bronchoalveolar lavege fluid (BALF), which was recovered at over 4 h after the inhalation of LPA. Infiltration in BALF was significantly inhibited by inhalation of Y-27632, an inhibitor of Rho-associated protein kinase (ROCK). LPA also increased superoxide production of eosinophils and neutrophils. In contrast, Y-27632 inhibited superoxide production. These findings suggest that LPA may contribute to infiltration and activation of inflammatory cells in bronchial asthma; furthermore, the Rho/ROCK-mediated pathway may be involved.
- 社団法人 日本薬理学会の論文
- 2003-01-01
著者
-
HASHIMOTO Terumasa
Department of Pharmacology, School of Pharmaceutical Sciences, Showa University
-
YAMASHITA Miyuki
Department of Pharmacology, School of Pharmaceutical Sciences, Showa University
-
OHATA Hisayuki
Department of Pharmacology, School of Pharmaceutical Sciences, Showa University
-
MOMOSE Kazutaka
Department of Pharmacology, School of Pharmaceutical Sciences, Showa University
-
Ohata H
Department Of Pharmacology School Of Pharmaceutical Sciences Showa University
-
Ohata Hisayuki
Department Of Pharmacology School Of Pharmaceutical Sciences Showa University
-
Momose Kazutaka
Department Of Pharmacology School Of Pharmaceutical Sciences Showa University
-
Momose Kazutaka
Department Of Phamacology School Of Pharmaceutical Sciences Showa University
-
Yamashita M
Department Of Pharmacology School Of Pharmaceutical Sciences Showa University
-
Yamashita Miyuki
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
-
Hashimoto Terumasa
Department Of Pharmacology School Of Pharmacy Shawa University
関連論文
- A Novel Embolic Model of Cerebral Infarction and Evaluation of Stachybotrys microspora Triprenyl Phenol-7 (SMTP-7), a Novel Fungal Triprenyl Phenol Metabolite
- Role of Rho-Associated Protein Kinase and Histamine in Lysophosphatidic Acid-Induced Airway Hyperresponsiveness in Guinea Pigs
- Effects of Papain on Isolaton of Single Smooth Muscle Cells from the Guinea Pig Longitudinal Ileum
- High-Glucose Enhances a Thromboxane A_2-Induced Aortic Contraction Mediated by an Alteration of Phosphatidylinositol Turnover
- Role of Rho-Associated Protein Kinase and Histamine in Lysophosphatidic Acid-Induced Airway Hyperresponsiveness in Guinea Pigs
- CYTOTOXICITY AND SUPPRESSION OF CALCIUM RESPONSE BY TRIBUTYLTIN-TREATMENT IN PRIMARY CULTURED HEPATOCYTES
- Suppression of Calcium Response to Hormonal Stimulation by Tributyltin in Primary Cultured Rat Hepatocytes
- Caspase Cascade Proceeds Rapidly After Cytochrome c Release From Mitochondria in Tumor Necrosis Factor-α-Induced Cell Death
- Simultaneous Real-Time Detection of Initiator- and Effector-Caspase Activation by Double Fluorescence Resonance Energy Transfer Analysis
- ROLE OF PHOSPHATIDIC ACID IN CONTRACTILE MECHANISMS OF SMOOTH MUSCLE
- Comparative Study of Calcium Ion Dynamics and Contractile Response in Rat Middle Cerebral and Basilar Arteries
- Lysophosphatidic Acid (LPA) Induces Plasma Exudation and Histamine Release in Mice via LPA Receptors
- Involvement of integrins in mechanical stress-induced local Ca^ influx, Ca^ spots, in the presence of lysophosphatidic acid in cultured lens epithelial cells
- Optical Bioimaging : From Living Tissue to a Single Molecule : Calcium Imaging in Blood Vessel In Situ Employing Two-Photon Excitation Fluorescence Microscopy
- Lysophosphatidic Acid Enhances In Vivo Infiltration and Activation of Guinea Pig Eosinophils and Neutrophils via a Rho/Rho-Associated Protein Kinase-Mediated Pathway
- Physiological and Pharmacological Role of Lysophosphatidic Acid as Modulator in Mechanotransduction
- Changes in Ca^ Signaling and Contractile Protein Isoforms in Smooth Muscle Cells from Guinea Pig Ileum during Culture
- Contractile Protein Isoforms of Single and Cultured Smooth Muscle Cells from Guinea Pig Ileum
- CHANGES IN CONTRACTILE RESPONSES OF SINGLE SMOOTH MUSCLE CELLS TO AGONISTS
- Inositol 1,4,5-Trisphosphate-Induced Contraction in Skinned Single Smooth Muscle Cells from Guinea-Pig Taenia Coli
- Identification and Expression of Frizzled-3 Protein in Rat Frontal Cortex After Antidepressant and Electroconvulsive Treatment
- STUDIES ON ISOLATED SMOOTH MUSCLE CELLS.VIII.^Ca-EFFLUX FROM SINGLE SMOOTH MUSCLE CELLS ISOLATED FROM TAENIA COLI OF GUINEA PIG
- STUDIES ON ISOLATED SMOOTH MUSCLE CELLS. IV. ISOLATION AND ACETYLCHOLINE-CONTRACTION OF SINGLE SMOOTH MUSCLE CELLS FROM TAENIA COLI OF GUINEA-PIG
- STUDIES ON ISOLATED SMOOTH MUSCLE CELLS. VII. RESPONSE TO AGONISTS AND THE CONTRACTION VELOCITY OF TAENIA COLI OF GUINEA PIG AND THE SINGLE SMOOTH MUSCLE CELLS
- A case of calcifying odontogenic cyst with numerous calcifications: immunohistochemical analysis
- ULTRASTRUCTURE OF SINGLE SMOOTH MUSCLE CELLS CONTRACTED BY CARBACHOL AND CALCIUM ION
- BINDING PROPERTIES OF HYDROPHILIC MUSCARINIC ANTAGONIST [^3H] NMeQNB IN THE SINGLE SMOOTH MUSCLE CELLS FROM GUINEA-PIG ILEUM
- Silica Gel-Dependent Production of Prodigiosin and Serrawettins by Serratia marcescens in a Liquid Culture
- STUDIES ON ISOLATED SMOOTH MUSCLE CELLS. V. CALCIUM AND CAFFEINE CONTRACTION OF SINGLE SMOOTH MUSCLE CELLS ISOLATED FROM TAENIA COLI OF GUINEA PIG
- A Novel Embolic Model of Cerebral Infarction and Evaluation of Stachybotrys microspora Triprenyl Phenol-7 (SMTP-7), a Novel Fungal Triprenyl Phenol Metabolite
- EFFECTS OF PLURONIC F-127 ON LOADING OF FURA 2/AM INTO SINGLE SMOOTH MUSCLE CELLS ISOLATED FROM GUINEA PIG TAENIA COLI
- Spontaneous Oscillations of Cytoplasmic Free Calcium Ion Concentration in Cultured Smooth Muscle Cells from Guinea Pig Ileum.
- Heterogeneous contractile responses of single smooth muscle cells from guinea pig ileum.
- The Relationship between Spontaneous Calcium Oscillations and Cell Proliferation in Cultured Smooth Muscle Cells.
- Effect of butyl 3-(1H-tetrazol-5-yl) oxanilate(MTB) on immunological or non-immunological histamine and SRS(-A) release from guinea-pig, monkey and human lung tissue.