STUDIES ON ISOLATED SMOOTH MUSCLE CELLS.VIII.^<45>Ca-EFFLUX FROM SINGLE SMOOTH MUSCLE CELLS ISOLATED FROM TAENIA COLI OF GUINEA PIG
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概要
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^<45>Ca-loaded single smooth muscle cells were prepared from taenia coli of guinea pig. The cells were perfused continuously with physiological salt solution on membrane filter with pore size of 12 μm and radioactivity in the effluent was determined. The ^<45>Ca-efflux curves consisted of two straight lines in semilogarithmic figure representing loosely and tightly bound calcium compartments with half time of 1.87±0.27 and 6.78±0.43min, respectively. The loosely bound calcium was supposed to be localized on plasma membrane. Size of the tightly bound ^<45>Ca was dependent on period for calcium-depletion of the preparation prior to ^<45>Ca-loading. The efflux was stimulated by acetylcholine and the stimulated efflux returned to the spontaneous efflux level instantly after removal of acetylcholine. This stimulation was abolished by atropine. Repetitive stimulation failed to increase ^<45>Ca-efflux while the rate of the spontaneous ^<45>Ca-efflux did not change significantly. These results suggested that, in taenia coli of guinea pig, at least three calcium compartments existed, loosely bound calcium, tightly bound calcium and more tightly bound calcium which can be released by acetylcholine, in addition to calcium in extracellular fluid. Advantages and disadvantages of using single smooth muscle cells for investigation of calcium movement and some technical improvement for determination of ^<45>Ca-efflux from single cells were described.
- 公益社団法人日本薬学会の論文
著者
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MOMOSE Kazutaka
Department of Pharmacology, School of Pharmaceutical Sciences, Showa University
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SATO Michiko
Department of Forest and Forest Products Science, Faculty of Agriculture, Kyushu University
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GOMI YASUO
Department of Pharmacology, Faculty of Pharmaceutical Science, Kanazawa University
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Gomi Yasuo
Department Of Pharmacology Faculty Of Pharmaceutical Science Kanazawa University
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Momose Kazutaka
Department Of Phamacology School Of Pharmaceutical Sciences Showa University
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Sato Michiko
Department Of Pharmacology Faculty Of Pharmaceutical Sciences Kanazawa University
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Sato Michiko
Department Of Forest And Forest Products Science Faculty Of Agriculture Kyushu University
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Sato Michiko
Department Of Biotechnology Tokyo University Of Agriculture And Technology
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