Heterogeneous contractile responses of single smooth muscle cells from guinea pig ileum.
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概要
- 論文の詳細を見る
Cells of guinea pig ileal longitudinal muscle layer were dispersed by treating the minced tissue with highly purified collagenase and papain. The cells were spindle shaped, 100 to 150μm long and 5 to 10μm wide. Contractility of the dispersed cells was determined by "cell adhesion method" resulting that the cells were contracted by carbachol in a dose-dependent manner, but sensitivity and degree of the contraction were different in each cell. Binding assay method resulted that Kd values did not change by treatment with collagenase and papain. These results indicate that contractility of each cell in the tissue is originally different and dispersion of the cells does not affect on the cell contractility.
- 日本平滑筋学会の論文
著者
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Fang Yang-il
Department Of Pharmacology School Of Pharmaceutical Sciences Showa University
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Momose Kazutaka
Department Of Phamacology School Of Pharmaceutical Sciences Showa University
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Fang Yang-Il
Department of Pharmacology, School of Pharmaceutical Sciences, Showa University
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KOMATSU Yoshimitsu
Department of Pharmacology, School of Pharmaceutical Sciences, Showa University
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