Splicing of a Bacterial Group II Intron from Bacillus megaterium Is Independent of Intron-Encoded Protein
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概要
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The splicing of a bacterial group II subclass B intron B.me.I1 from Bacillus megaterium chromosomes was investigated. RT-PCR and nucleic acid hybridization methods were used to understand the role of the intron-encoded protein (IEP) in the splicing of B.me.I1. An in vivo assay showed that the splicing occurred in the absence of IEP. An in vitro assay showed that B.me.I1 was spliced under conditions similar to those of the intracellular environment with no help from other biological molecules. Because all group II introns previously reported needed IEPs for their splicing in vivo, our results suggest that B.me.I1 is an "actual" self-splicing group II intron. This is also the first report to recognize the existence of group II introns that independently splice mRNA in vivo. The self-splicing of a bacterial intron may support that eukaryotic spliceosomal introns originated in bacterial genomes.
- 日本微生物生態学会の論文
著者
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Chien Mei-Fang
Faculty of Engineering, Tohoku Gakuin University
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Tosa Saeko
Faculty Of Engineering Tohoku Gakuin University
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Endo Ginro
Faculty Of Engieering Tohoku Gakuin University
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Chien Mei-fang
Faculty Of Engineering Tohoku Gakuin University:(present Office)center For Marine Environmental Stud
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Huang Chieh-chen
Department Of Dermatology Shin-kong Wu Ho-su Memorial Hospital
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