Isolation of cDNA for Pea Phytochrome Using an Expression Vector
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概要
- 論文の詳細を見る
Partially purified phytochrome mRNA was obtained from etiolated pea epicotyls by polyribosome immunoprecipitation or by size fractionation of total poly(A)^+RNA, and used for the synthesis of double-stranded complementary DNA (cDNA). cDNA libraries were constructed using an Escherichia coli expression vector, pUC9, and screened for phytochrome cDNA by colony immunological assay. Nine colonies were found to produce a 27 kDa polypeptide that was reactive to both polyclonal and monoclonal anti-pea phytochrome antibodies. The plasmids from these colonies contained cDNA inserts of 1.2 or 2.0 kbp. Hybridization-arrest translation assay verified that the cDNA clones contained a sequence coding for phytochrome polypeptide. RNA blot hybridization analysis indicated that the cDNA hybridized to a 4.1 kb poly(A)^+RNA in dark-grown pea.
- 日本植物生理学会の論文
著者
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SATO Naoki
Department of Neurosurgery, Osaka Neurological Institute
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Furuya Masaki
Frontier Research Programs Riken Institute
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Furuya Masaki
Frontier Research Programs Riken
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Furuya Masaki
Department Of Botany Faculty Of Science University Of Tokyo
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Nagatani Akira
Division of Biological Regulation, National Institute for Basic Biology
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Sato N
Department Of Botany Faculty Of Science University Of Tokyo
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Nagatani Akira
Division Of Biological Regulation National Institute For Basic Biology
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Komeda Y
Hokkaido Univ. Sapporo Jpn
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Komeda Yoshibumi
Frontier Research Programs Riken Institute
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Komeda Yoshibumi
Division Of Bilogical Sciences Graduate School Of Science Hokkaido University
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Komeda Yoshibumi
Molecular Genetics Research Laboratory The University Of Tokyo:(present)division Of Biological Scien
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Komeda Yoshibumi
Molecular Genetics Research Laboratory The University Of Tokyo
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TOMIZAWA Ken-ichi
RITE, Kizu
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Tomizawa Ken-ichi
Department of Biology, Faculty of Science, University of Tokyo
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Iino Tetsuo
Department of Biology, Faculty of Science, University of Tokyo
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Tomizawa Ken-ichi
Frontier Research Programs Riken Institute
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Tomizawa Ken-ichi
Rite Kizu
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Tomizawa Ken-ichi
Department Of Biology Faculty Of Science Toyama University:(present Permanent)botany Department Facu
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Sato N
Saitama Univ. Urawa Jpn
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Iino T
Univ. Tokyo Tokyo Jpn
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Sato Naoki
Departentm Of Chemisty Faculty Of Science Shinshu University
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