Maternal Effects of mtol Mutation, That Causes Overaccumulation of Soluble Methionine, on the Expression of a Soybean β-Conglycinin Gene Promoter-GUS Fusion in Transgenic Arabidopsis thaliana : GROWTH AND DEVELTOPMENT : GENES STRUCTURE AND EXPRESSION

概要

論文の詳細を見る
β-Conglycinin, the 7S seed storage protein of soybean (Glycine max [L.] Merr.), is comprised mainly of three subunits, designated α, α' and p. Expression of the gene encoding the P subunit i~ unique because its expression has been shown to be down-regulated by exogenously applied L-methionine in immature soybean cotyledon cultures in vitro. Arabidopsis thaliana strain carrying a mto1-1 mutation overaccumulates soluble methionine. By using this mutant, we analyzed the effects of methionine on expression of the P subunit gene in vivo. Reciprocal crosses were made between the mot1-1 mutant and a transgenic A. thaliana strain, designated SNTβ3, which carries a β-glucuronidase (GUS) reporter gene under the control of the promoter region of the P subunit gene. Analysis of GUS activity in F_1 seeds indicated that the GUS activity was dramatically repressed when the mot1-1 mutant plants were used as female parents. We constructed a strain which carries both the transgene and mto1-1 mutation in the homozygous state. Analyses of the GUS activity in seeds of this double homozygous strain indicated that the GUS activity was repressed to 2.5% of control by introduction of the mot1-1 mutation. These results indicate that the β subunit gene promoter activity in seeds is down-regulated by maternal genotype and suggest that soluble methionine, or its mobile metabolite, is translocated from mother plants to repress β subunit gene expression in seeds.
日本植物生理学会の論文

Maternal Effects of mtol Mutation, That Causes Overaccumulation of Soluble Methionine, on the Expression of a Soybean β-Conglycinin Gene Promoter-GUS Fusion in Transgenic Arabidopsis thaliana : GROWTH AND DEVELTOPMENT : GENES STRUCTURE AND EXPRESSION

スポンサーリンク

概要

著者

関連論文

スポンサーリンク