Differential Regulation of Soybean Seed Storage Protein Gene Promoter-GUS Fusions by Exogenously Applied Methionine in Transgenic Arabidopsis thaliana : GROWTH AND DEVELTOPMENT : GENES STRUCTURE AND EXPRESSION
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概要
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Expression of the gene encoding the β subunit of β-conglycinin, the 7S seed storage protein of soybean (Glycine max [L.] Merr.), has been shown to be down-regulated by exogenously applied L-methionine in in vitro soybean cotyledon cultures, whereas expression of the gene en-coding the α' subunit is not affected. We constructed transgenic Arabidopsis thaliana (L.) Heynh. carrying the β-glucuronidase (GUS) reporter gene under the control of the promoter regions from either the α' or β subunit gene. An in vitro culture system of immature siliques of A. thaliana was developed for exogenous application of L-methionine to immature seeds. Expression of the β sub-unit gene promoter-GUS fusion was down-regulated by the application of L-methionine, and this response was reversible. As an alternative way to apply L-methionine, A. thaliana plants were grown on rockwool and irrigated with L-methionine-supplemented medium. GUS activity driven by the β subunit gene promoter was repressed by L-methionine, and the degree of repression depended on the concentration of L-methionine in the medium. On the other hand, the α' subunit gene promoter-GUS fusion did not show such a response. These results indicate that A. thaliana has a system to differentially regulate the expression of the β-conglycinin genes by L-methionine at the level of transcription. This work establishes a system to genetically analyze plants' responses to nutritional stimuli.
- 日本植物生理学会の論文
著者
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CHINO Mitsuo
Department of Biological Production, Faculty of Bioresource Sciences, Akita Prefectural University
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FUJIWARA Toru
Department of Applied Biological Chemistry, The University of Tokyo
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Yokota Hirai
Department of Applied Biological Chemistry Graduate School of Agricuitural and Life Sciences, The Un
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Yokota Hirai
Department Of Applied Biological Chemistry Graduate School Of Agricuitural And Life Sciences The Uni
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Yokota Hirai
Laboratory Of Molecular Biology And Biotechnology In Research Center Of Medicinal Resources Faculty
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Naito S
Hokkaido Univ Sapporo Jpn
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Naito S
Hokkaido Univ. Sapporo Jpn
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Naito Satoshi
Dept. Appl. Biosci. Fac. Agric. Hokkaido Univ. Sapporo
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Chino Mitsuo
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Komeda Y
Hokkaido Univ. Sapporo Jpn
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Komeda Yoshibumi
Frontier Research Programs Riken Institute
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Komeda Yoshibumi
Division Of Bilogical Sciences Graduate School Of Science Hokkaido University
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Komeda Yoshibumi
Molecular Genetics Research Laboratory The University Of Tokyo:(present)division Of Biological Scien
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Komeda Yoshibumi
Molecular Genetics Research Laboratory The University Of Tokyo
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Naito Satoshi
Molecular Genetics Research Laboratory, The University of Tokyo
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Fujiwara Toru
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Goto Koji
Molecular Genetics Research Laboratory The Uiversity Of Tokyo
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Naito Satoshi
Molecular Genetics Research Laboratory The University Of Tokyo:(present)department Of Applied Biosci
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内藤 哲
北大院・生命
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Naito Satoshi
Dept Appl. Biosci. Fac. Agric. Hokkaido Univ.
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