Effects of Sulfate Concentrations on the Expression of a Soybean Seed Storage Protein Gene and its Reversibility in Transgenic Arabidopsis thaliana : GROWTH AND DEVELOPMENT : ENVIRONMENTAL AND STRESS RESPONSES

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Transgenic expression of genes encoding the a' and β subunits of β-conglycinin, one of the major seed storage proteins of soybean (Glycine max [L.] Merr.), was analyzed in Arabidopsis thaliana (L.) Heynh. under conditions of sulfate deficiency. Temporal patterns of expression of both the intact β subunit gene and the β subunit gene promoter fused to the β-glucuronidase (GUS) gene are similar in soil-less cultures using rockwool, suggesting that the response to sulfate deficiency is regulated mainly at the level of transcription. In hydroponic cultures with various concentrations of sulfate, expression of both the intact β subunit gene and the β subunit gene promoter-GUS fusion gene were negatively correlated to increased sulfate concentrations in the culture medium. Transfer of transgenic A . thaliana plants carrying the β subunit gene promoter-GUS fusion from sulfate-deficient to sulfate-sufficient control medium caused GUS activity in developing siliques to be repressed within two days. A reverse shift, where the plants were transferred from the control to sulfate-deficient medium, caused GUS activity to become higher than that in seeds of the control plants within two days. These results indicate that the expression of the β subunit gene promoter responds rapidly to changes of sulfate availability.
日本植物生理学会の論文