Partial Enrichment of Pea Phytochrome mRNA by Gel Electrophoresis and Transfer to Poly (U)-Paper

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概要

• 論文の詳細を見る

• Total cellular poly(A)^+RNA from etiolated pea epicotyls was fractionated by urea-agarose gel electrophoresis and recovered by transfer to poly(U)-paper. Phytochrome mRNA activity was detected in RNA fractions that migrated slightly slower than the 25S rRNA in the electrophoresis. A single run of this fractionation enriched the phytochrome mRNA content 20-fold. This technique together with hybridization on poly(U)-paper made it possible to compare the mobilities of translatable and hybridizable phytochrome mRNA. The advantages of the present method of mRNA fractionation in preparing enriched mRNA and identifying cDNA clone are discussed.

• 日本植物生理学会の論文

著者

• SATO Naoki

  Department of Neurosurgery, Osaka Neurological Institute

• Furuya Masaki

  Frontier Research Programs Riken

• Furuya Masaki

  Department Of Botany Faculty Of Science University Of Tokyo

• Sato N

  Department Of Botany Faculty Of Science University Of Tokyo

• Komeda Y

  Hokkaido Univ. Sapporo Jpn

• Komeda Yoshibumi

  Frontier Research Programs Riken Institute

• Komeda Yoshibumi

  Division Of Bilogical Sciences Graduate School Of Science Hokkaido University

• Komeda Yoshibumi

  Molecular Genetics Research Laboratory The University Of Tokyo:(present)division Of Biological Scien

• Komeda Yoshibumi

  Molecular Genetics Research Laboratory The University Of Tokyo

• TOMIZAWA Ken-ichi

  RITE, Kizu

• Tomizawa Ken-ichi

  Department of Biology, Faculty of Science, University of Tokyo

• Tomizawa Ken-ichi

  Frontier Research Programs Riken Institute

• Tomizawa Ken-ichi

  Rite Kizu

• Tomizawa Ken-ichi

  Department Of Biology Faculty Of Science Toyama University:(present Permanent)botany Department Facu

• Sato N

  Saitama Univ. Urawa Jpn

• Sato Naoki

  Departentm Of Chemisty Faculty Of Science Shinshu University

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