Analysis of Tissue-Specific Expression of Arabidopsis thaliana HSP90-Family Gene HSP81 : GROWTH AND DEVELTOPMENT : ENVIRONMENTAL AND STRESS RESPONSES
スポンサーリンク
概要
- 論文の詳細を見る
We have isolated three HSP90-family genes from Arabidopsis: HSP81-1 which is heat-inducible, and HSP81-2 and -3 which are highly expressed under normal growth temperatures. Northern blot analysis and RNase protection analysis, using gene specific probes, showed that HSP81-2 and -3 mRNA were present in all tissues and abundant in roots, floral bud clusters, and flowers at 22℃. A small amount of HSP81-1 mRNA was detected only in roots. In situ hybridization and histochemical analysis using transgenic plants carrying chimeric gene fusions, with an HSP81 promotor region fused to a β-glucuronidase (GUS) gene, confirmed these results. At 22℃, high GUS activity was observed in the root apical meristems, pollen and tapeta in HSP81-2::GUS and HSP81-3::GUS transgenic plants, while only branches of the root in HSP81-1::GUS transgenic plants expressed high GUS activity. After 2 hours of 35℃ treatment, extensively high GUS activity was observed in all tissues in HSP81-1::GUS transgenic plants, while elevated but tissue specific expression was observed in HSP81-2 and -3 transgenic plants. Exogenous application of various chemicals such as ABA, GA_3, kinetin, IAA, NaCl, and mannitol revealed that 10 mM IAA and 0.1 M NaCl significantly enhanced the accumulation of HSP81-2 and -3 transcripts. Only a slight response to IAA was observed in HSP81-1 mRNA accumulation at 22℃; the increase was possibly caused by a novel pathway other than heat-shock-response pathway.
- 日本植物生理学会の論文
著者
-
Yabe Naoto
Molecular Genitics Research Laboratory The University Of Tokyo:(present)department Of Plant Sciences
-
Komeda Y
Hokkaido Univ. Sapporo Jpn
-
Komeda Yoshibumi
Frontier Research Programs Riken Institute
-
Komeda Yoshibumi
Division Of Bilogical Sciences Graduate School Of Science Hokkaido University
-
Komeda Yoshibumi
Molecular Genetics Research Laboratory The University Of Tokyo
-
Takahashi Taku
Molecular Genetics Research Laboratory University Of Tokyo
関連論文
- Characterization and Intracellular Distribution of Pea Phytochrome I Polypeptides Expressed in E. coli
- Regulated Expression of a Gene-Fusion Product Derived from the Gene for Phytochrome I from Pisum sativum and the uidA Gene from E. coli in Transgenic Petunia hybrida
- Isolation of cDNA for Pea Phytochrome Using an Expression Vector
- Partial Enrichment of Pea Phytochrome mRNA by Gel Electrophoresis and Transfer to Poly (U)-Paper
- A Xylanase, AtXyn1, is Predominantly Expressed in Vascular Bundles, and Four Putative Xylanase Genes were Identified in the Arabidopsis thaliana Genome
- An RNA-Binding Protein, AtRBP1, Is Expressed in Actively Proliferative Regions in Arabidopsis thaliana
- Structural and Functional Characterization of the Intergenic Spacer Region of the rDNA in Daucus carota
- Three Protein Fractions That Bind to a Narrow Region of the Promoter of the Ribosomal RNA Gene in Vicia faba : GENE STRUCTURE AND EXPRESSION : PROTEINS, ENZYMES AND METABOLISM
- Electrophoretic Analysis of Florally-Evoked Meristems of Pharbitis nil Choisy cv. Violet
- Tissue-Dependent Expression of the Rice wx^+ Gene Promoter in Transgenic Rice and Petunia : GROWTH AND DEVELOPMENT : GENE STRUCTURE AND EXPRESSION
- Differential Regulation of Soybean Seed Storage Protein Gene Promoter-GUS Fusions by Exogenously Applied Methionine in Transgenic Arabidopsis thaliana : GROWTH AND DEVELTOPMENT : GENES STRUCTURE AND EXPRESSION
- Flowering of Arabidopsis cop1 Mutants in Darkness
- Highly Repetitive Tandem Arrays of a Short 32 bp Unit in the Pharbitis nil Genome : the RsaI Family
- Phenotypic Characterization and Molecular Mapping of anacaulis2 Mutant of Arabidopsis thaliana with Flower Stalks of Much Reduced Length : GROWTH AND DEVELOPMENT : STRUCTURE AND FUNCTION OF CELLS
- Cloning and Characterization of an L1 Layer-Specific Gene in Arabidopsis thaliana
- Analysis of Tissue-Specific Expression of Arabidopsis thaliana HSP90-Family Gene HSP81 : GROWTH AND DEVELTOPMENT : ENVIRONMENTAL AND STRESS RESPONSES
- Expression in Yeast of a Fusion Gene Composed of the Promoter of a Heat-Shock Gene from Arabidopsis and a Bacterial Gene for β-Glucuronidase
- Heat-Shock Induced Accumulation of Extremely Long Transcripts Containing the rDNA Intergenic Spacer in Arabidopsis
- Maternal Effects of mtol Mutation, That Causes Overaccumulation of Soluble Methionine, on the Expression of a Soybean β-Conglycinin Gene Promoter-GUS Fusion in Transgenic Arabidopsis thaliana : GROWTH AND DEVELTOPMENT : GENES STRUCTURE AND EXPRESSION
- Formation of Corymb-like Inflorescences Due to Delay in Bolting and Flower Development in the corymbosa2 Mutant of Arabidopsis
- CHARACTERIZATION OF THE SPERMIDINE SYNTHASE GENE FAMILY IN ARABIDOPSIS THALIANA
- MOLECULAR GENETIC ANALYSIS OF CRM2; AN ARABIDOPSIS MUTANT WITH CORYMB-LIKE INFLORESCENCE
- MOLECULAR GENETIC ANALYSIS OF corymbosa2,AN ARABIDOPSIS MUTANT WITH CORYMB-LIKE INFLORESCENCE
- POSITIONAL CLONING OF ACAULIS5, A GENE REQUIRED FOR INTERNODE FLONGATION IN Arabidopsis thaliana.
- Development of Inflorescences in Arabidopsis thaliana
- THE ROLE OF ACAULIS5 IN THE INTERNODAL GROWTH OF Arabidopsis thaliana
- CHARACTERIZATION OF ARABIDOPSIS MUTANTS CARRYING THE CORYMBOSE INFLORESCENCE