Electrophoretic Analysis of Florally-Evoked Meristems of Pharbitis nil Choisy cv. Violet
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概要
- 論文の詳細を見る
Seedlings of Pharbitis nil Choisy cv. Violet were subjected to a single SD (floral induction) or NB (inhibition of induction) treatment. Soluble proteins were extracted from the apical region of seedlings just before the treatment or at various times after the treatment and were analyzed by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) to identify the changes specifically involved in floral induction. The patterns of polypeptides were almost identical between apices of short day-treated plants (SD-plants) and night break-treated plants (NB-plants), and quantitative differences were found in five polypeptide spots. Intensities of three of these polypeptide spots increased gradually or transiently after SD treatment. The remaining two spots in-creased in intensities after NB treatment. A fairly consistent results were obtained by a similar analysis of proteins labelled in vivo. These differences were detected at relatively early stages of flower formation, and some of them were retained or even magnified at a later stage. These findings suggest that there are remarkably subtle differences between the complements of proteins in the evoked and non-evoked apical meristems, and that a few consistent quantitative differences are clearly apparent at the relatively early stages of formation of flowers.
- 日本植物生理学会の論文
著者
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Komeda Yoshibumi
Molecular Genetics Research Laboratory The University Of Tokyo:(present)division Of Biological Scien
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Komeda Yoshibumi
Molecular Genetics Research Laboratory The University Of Tokyo
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Araki T
Graduate School Of Bio-applications And Systems Engineering Tokyo University Of Agriculture And Tech
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Araki Takashi
Molecular Genetics Research Laboratory, University of Tokyo
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