Regulated Expression of a Gene-Fusion Product Derived from the Gene for Phytochrome I from Pisum sativum and the uidA Gene from E. coli in Transgenic Petunia hybrida

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The 5'-upstream region (2.4 kb) of the gene for phytochrome I from Pisum sativum (phyI) was fused to the uidA gene from Escherichia coli that encodes β-glucuronidase (GUS). The resulting PHY-GUS fusion was introduced into Petunia hybrida and was used as a reporter of the expression of the phyI gene which was recognized by GUS activity. The PHY-GUS fusion was expressed at a relatively high level when transgenic plants were grown in the dark, while leaves and stems of light-grown plants showed background activity. Flowers of light-grown plants were shown to have significant levels of GUS activity but roots did not have such activity. When light-grown transgenic plants were transferred to the dark, they expressed the activity at levels that corresponded to those of dark-grown plants. Light treatment prior to growth in darkness revealed red/far-red reversibility of recovery of the activity. Thus, the 2.4-kb fragment from the 5' region of the phyI gene carries the information necessary for the light-repressible autoregulation.
日本植物生理学会の論文