Isolation of the Red-light-absorbing Form of Phytochrome from Light-grown Pea Shoots
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概要
- 論文の詳細を見る
Phytochrome was extracted from both light-grown and dark-grown shoots of Pisum and partially purified by brushite chromatography and ammonium sulfate fractionation. About 160-270 ng of phytochrome per g green tissue extracted was recovered after the partial purification while about 5.1-8.6 μg of phytochrome per g etiolated tissue was recovered. Only the red-light-absorbing form of phytochrome was detected in extracts prepared from both light- and dark-grown tissue, even though the light-grown tissue was harvested in daylight and purification was done entirely at 0-4℃ with only a dim green safe light. No significant differences were found between phytochrome purified from green and etiolated tissues, either in their spectral properties or in their immunochemical reactivity against antietiolated-zucchini-phytochrome serum.
- 日本植物生理学会の論文
著者
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Furuya Masaki
Department Of Botany Faculty Of Science University Of Tokyo
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Shimazaki Yukio
Biology Department, Faculty of Science, University of Tokyo
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Moriyasu Yuji
Biology Department, Faculty of Science, University of Tokyo
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Pratt Lee
Biology Department, Faculty of Science, University of Tokyo
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Furuya Masaki
Biology Department, Faculty of Science, University of Tokyo
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Pratt Lee
Biology Department Faculty Of Science University Of Tokyo
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Moriyasu Yuji
Biology Department Faculty Of Science University Of Tokyo
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Shimazaki Yukio
Biology Department Faculty Of Science University Of Tokyo
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