Electrostatic Binding of Proteins and Phytochrome to Differently Charged Liposomes
スポンサーリンク
概要
- 論文の詳細を見る
The sign and magnitude of the surface charge of liposomes containing electrostatically neutral lecithin and cholesterol was altered by incremental additions of dicetyl phosphate or stearylamine. Such liposomes instantaneously bound authentic proteins at 0℃ only when they had electrostatically opposite charges; 1 M NaCl inhibited the binding. The amount of protein bound was dependent upon the concentration of protein and the charge of liposomes. Phytochrome in a crude extract of etiolated pea (Pisum sativum cv. Alaska) shoots could bind equally well to liposomes with either positive or negative charges irrespective of P_R and P_<FR> both of which showed no spectral distortion. Both P_R and P_<FR> of purified pea phytochrome bound entirely to positively charged liposomes but partially to negatively charged ones. In this association both P_R and P_<FR> became pelletable at similar rates. Absorption spectra of liposome-bound P_R Showed a small blue shift and then a crucial spectral distortion after red-light irradiation.
- 日本植物生理学会の論文
著者
-
Furuya Masaki
Department Of Botany Faculty Of Science University Of Tokyo
-
Furuya Masaki
Department Of Botany Glasgow University Department Of Biology. Facultv Of Science. University Of Tok
-
Freer.H J
Department of Microbiology, Glasgow University
-
Ellis A
Department of Microbiology, Glasgow University
-
Yamamoto.T Kotaro
Department of Biology. Facultv of Science. University of Tokyo,Division of Biological Regulation. Na
-
Yamamoto.t Kotaro
Department Of Biology. Facultv Of Science. University Of Tokyo Division Of Biological Regulation. Na
-
Yamamoto.t Kotaro
Department Of Biology Faculty Of Science University Of Tokyo:(present)division Of Bioligical Regulat
-
Freer.h J
Department Of Microbiology Glasgow University
-
Ellis A
Department Of Microbiology Glasgow University
関連論文
- Production and Characterization of Monoclonal Antibodies Which Distinguish Different Surface Structues of Pea (Pisum sativum cv. Alaska) Phytochrome
- Production and Characterization of Monoclonal Antibodies to Rye (Secale cereale) Phytochrome
- Intracellular Localization and Dichroic Orientation of Phytochrome in Plasma Membrane and/or Ectoplasm of a Centrifuged Protonema of Fern Adiantum capillus-veneris L.
- Intracellular Photoreceptive Site for Polarotropism in Protonema of the Fern Adiantum capillus-veneris L.
- Apical Growht of Protonemata in Adiantum capillus-veneris-2-Action Spectra for the Induction of Apical Swelling and the Intracellular Photoreceptive Site
- Dichroic Orientation of Phytochrome Intermediates in the Pathway fromn P_R to P_ as Analyzed by Double Laser Flash Irradiations in Polarotropism of Adiantum Protonemata
- Dark transformations of phytochrome in cotyledons of Pharbitis nil
- Electrostatic Binding of Proteins and Phytochrome to Differently Charged Liposomes
- Photocontrol of Nuclear DNA Replication in Chattonella antiqua (Raphidophyceae)
- Effects of Gabaculine on Phytochrome Synthesis during Imbibition in Embryonic Axes of Pisum sativum L.
- Isolation of cDNA for Pea Phytochrome Using an Expression Vector
- Temporal Changes in Swimming Direction during the Phototactic Orientation of Individual Cells in Cryptomonas sp.
- Phototransformation of the Red-Light-Absorbing Form to the Far-Red-Light-Absorbing Form of Phytochrome in Pea Epicotyl Tissue Measured by a Multichannel Transient Spectrum Analyser
- Involvement of Microtubules on Nuclear Positioning during Apical Growth in Adiantum protonemata
- Role of the Photoperiod Preceding a Flower-Inductive Dark Period in Dark-Grown Seedlings of Pharbitis nil Choisy
- Phytochrome-Mediated Control of Absorbance Decrease at 630 nm in Primary Roots of Zea mays
- Changes in Organelle Movement in the Nuclear Region during the Cell Cycle of Adiantum Protonema
- Action Spectrum between 250 and 800 Nanometers for the Photoinduced Inhibition of Spore Germination in Pteris vittata
- Studies on the Photoreceptors for the Promotion and Inhibition of Flowering in Dark-Grown Seedlings of Pharbitis nil Choisy
- Isolation and Identification of Diacylglyceryl-O-4'-(N,N,N-trimethyl)-homoserine from the Fern Adiantum capillus-veneris L.
- Spectral Properties of Chromophore-Containing Fragments Prepared from Pea Phytochrome by Limited Proteolysis
- A Digital Image Processing Technique for the Analysis of Particle Movements : Its Application to Organelle Movements during Mitosis in Adiantum Protonemata
- Effects of Calcium and Potassium Ions on Phototaxis in Cryptomonas
- Phototransformation of the Far-red Light-absorbing Form of Large Pea Phytochrome by Laser Flash Excitation
- Spectral Dependence of Night-break Effect on Photoperiodic Floral Induction in Lemna paucicostata 441
- Identification of a Triterpenoid Saponin in Etiolated Pea Shoots as Phytochrome Killer
- Isolation of the Red-light-absorbing Form of Phytochrome from Light-grown Pea Shoots
- Apical Growth of Protonemata in Adiantum capillus-veneris IV. Phytochrome-Mediated Induction in Non-Growing Cells
- Phototransformation of the red-light-absorbing form of undegraded pea phytochrome by laser flash excitation
- Photoreversible and photoirreversible absorbance changes in the red and far-red spectral regions in Zea primary roots
- Partial Enrichment of Pea Phytochrome mRNA by Gel Electrophoresis and Transfer to Poly (U)-Paper
- Synthesis of Translatable mRNA for Phytochrome during Imbibition in Embryonic Axes of Pisum sativum L.
- Phototaxis in Cryptomonas sp. under condition suppressing photosynthesis
- Effects of enzymatically digested microsome fractions on red-light-enhanced pelletability of pea phytochrome in vitro in the presence of calcium ion
- Isolation of a naturally occurring inhibitor for dark Pfr reversion from etiolated Pisum epicotyls
- Action spectrum of phototaxis in a cryptomonad alga, Cryptomonas sp.
- Perithecial formation in Gelasinospora reticulispora III. Inhibitory effects of near-UV and blue light during the inductive dark period
- Perithecial formation in Gelasinospora reticulispora II. Promotive effects of near-ultraviolet and blue light after dark incubation