Characterization of Green Tissue-Specific Phytochrome Isolated Immunochemically from Pea Seedlings

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Phytochrome was isolated and purified from light-grown pea (Pisum sativum) seedlings and compared with that from dark-grown seedlings in terms of spectral and immunochemical properties. Approximately 40% of phytochrome in the brushite eluate prepared from light-grown pea tissue bound with a monoclonal anti-pea phytochrome antibody (mAP3), but the remaining 60% did not. Both phytochrome fractions showed a typical photoreversible absorbance change after alternate red and far-red actinic irradiations, which was similar to that of phytochrome from etiolated pea tissue. The peptide mapping of the mAP3-bound phytochrome from light-grown tissue was essentially the same as that of the mAP3-bound phytochrome from etiolated tissue. However, the digestion pattern of the phytochrome that was prepared from light-grown tissue but which did not bind to mAP3 was obviously different from that of mAP3-bound phytochrome. Polyclonal anti-pea phytochrome antibodies and mAP5 and 10, however, bound to both the phytochromes. These results suggest that light-grown tissue contains two phytochrome pools which are distinct from each other with respect to the primary structure of the phytochrome polypeptide but which share a few common determinant sites.
日本植物生理学会の論文