Chemical Genetics Reveal the Novel Transmembrane Protein BIL4, Which Mediates Plant Cell Elongation in Brassinosteroid Signaling

スポンサーリンク

概要

• 論文の詳細を見る

• Steroid hormones are conserved between animals and plants as signaling molecules to control growth and development. Plant steroid hormones, brassinosteroids (BRs), appear to play an important role in plant cell elongation. BRs bind to leucine-rich repeat kinase BRASSINOSTEROID-INSENSITIVE 1 (BRI1) localized to the plasma membrane, activate transcription factors in collaboration with cytosolic kinases and phosphatases, and regulate BR-responsive gene expression, but the details regarding the BR signaling pathway from perception to nuclear events remain unknown. In this study we used chemical genetics to identify an evolutionarily conserved transmembrane protein, Brz-insensitive-long hypocotyls 4 (BIL4), and demonstrated its role as a critical component of plant cell elongation occurring upon BR signaling. A dominant mutation, bil4-1D, showed cell elongation in the presence of the BR-specific inhibitor Brz. Brz suppresses expression of the BIL4 gene in wild-type plants, and overexpression of BIL4 in bil4-1D suppresses the BR deficiency caused by Brz. Our results indicate that BIL4 mediates cell elongation on BR signaling.

• 社団法人 日本農芸化学会の論文

著者

• MATSUI Minami

  Plant Function Exploration Team, Plant Functional Genomics Research Group

• NAKANO Takeshi

  Plant Functions Lab., RIKEN Inst.

• NAKAZAWA Miki

  Plant Function Exploration Team, Plant Functional Genomics Research Group, Riken Genomics Sciences C

• Yamagami Ayumi

  Plant Chemical Biology Research Unit, RIKEN Advanced Science Institute

• TUJIMOTO Masafumi

  Laboratory of Cellular Biochemistry, RIKEN Advanced Science Institute

• SAKUTA Masaaki

  Department of Biology, Ochanomizu University

• ASAMI Tadao

  Laboratory of Cellular Biochemistry, RIKEN Advanced Science Institute

関連論文

• ISOLATION AND CHARACTERIZATION OF SUPPRESSORS OF cop1
• Screnning of cytokinin biosynthesis inhibitors
• A novel albino mutant, alth deficient in chloroplast gene expression.
• Bipolar Localization of Putative Photoreceptor Protein for Phototaxis in Thermophilic Cyanobacterium Synechococcus elongatus
• Analysis of phototropic curvature in npl1 by phytochrome modulation
• Expression and Interaction Analysis of Arabidopsis Skp1-Related Genes
• Chemical Genetics Reveal the Novel Transmembrane Protein BIL4, Which Mediates Plant Cell Elongation in Brassinosteroid Signaling
• Cytokinin inducible genes in culture green tobacco cells.
• Analysis of phyotohormone contents in tobacco CND41 antisense transformants.
• Analyzing the function of a 41 kD chloroplast-nucleoid DNA-binding protein (CND41) by F2 progeny of antisense-transgenic tobacco
• LOCALIZATION OF FLAVONOID 3'-HYDROXYLASE AND FLAVONOID, AND ANTIOXIDANT ACTIVITY OF TISSUES IN SOYBEAN
• TFPD, A NOVEL PROTEIN WHICH BINDS TO THE TRANSCRIPTION ENHANCER OF CHLOROPLAST psbD LRP
• Arabidopsis obtusifoliol 14α-demethylase (CYP51) in sterol biosynthetic pathway
• POSSIBLE ROLE OF CND41 IN DEGRADATION OF RUBISCO
• Protease activity of CND41, a chloroplast DNA-binding protein
• Cloning Genomic DNA Encoding Apple Polyphenol Oxidase and Comparison of the Gene Product in Escherichia coli and in Apple
• Expression and Induction of Polyphenol Oxidase in Apple (Malus pumila) Cell Culture
• Why does the Caryphyllales not produce anthocyanins?
• Classification and Expression Analysis of Arabidopsis F-Box-Containing Protein Genes
• Dihydroflavonol 4-reductase cDNA from non-Anthocyanin-Producing Species in the Caryophyllales
• Functinal analysis of cig (cytokinin inducible gene)
• PURIFICATION AND CHARACTERIZATION OF CND41 FROM CULTURED TOBACCO CELL
• Analysing the function of a 41 kD chloroplast-nucleoid DNA-binding protein (CND41) by cultured cells from antisense-transgenic tobacco
• CLONING OF A NOVEL PROTEIN WHICH BINDS TO THE TRANSCRIPTION ENHANCER OF CHLOROPLAST psbD LRP BY ONE-HYBRID SYSTEM
• Production and characterization of a large population of cDNA-overexpressing transgenic rice plants using Gateway-based full-length cDNA expression libraries
• DEAR1, a transcriptional repressor of DREB protein that mediates plant defense and freezing stress responses in Arabidopsis
• Chemical Genetic Screening Identifies a Novel Inhibitor of Parallel Alignment of Cortical Microtubules and Cellulose Microfibrils
• Functional Differentiation of Lotus japonicus TT2s, R2R3-MYB Transcription Factors Comprising a Multigene Family
• Comparative Analysis of the Triplicate Proathocyanidin Regulators in Lotus japonicus
• Analysis of circadian rhythm in Arabidopsis LKP2 transgenic plants
• Phenome Analysis in Plant Species Using Loss-of-Function and Gain-of-Function Mutants
• The AtXTH28 Gene, a Xyloglucan Endotransglucosylase/Hydrolase, is Involved in Automatic Self-Pollination in Arabidopsis thaliana
• Structure of GDP/GTP exchange factor homolog which expression was induced by cytokinin in cultured green tobacco cells
• Arabidopsis MBF1s Control Leaf Cell Cycle and its Expansion
• Characterization of SBZ1, a soybean bZIP protein that binds to the chalcone synthase gene promoter
• Chemical Genetics Reveal the Novel Transmembrane Protein BIL4, Which Mediates Plant Cell Elongation in Brassinosteroid Signaling
• Cytokinin-induced Gene Expression in Cultured Green Cells of Nicotiana tabacum Identified by Fluorescent Differential Display
• Transcriptional control of the dihydroflavonol 4-reductase multigene family in Lotus japonicus
• Efficient Yeast One-/Two-Hybrid Screening Using a Library Composed Only of Transcription Factors in Arabidopsis thaliana

もっと見る 閉じる

スポンサーリンク