Characterization of CDC48 in Allium cepa
スポンサーリンク
概要
- 論文の詳細を見る
Onion, Allium cepa, is a model plant for experimental observation of mitotic cell division in practice of fundamental biology, because its chromosome is big and easy to observe. In order to develop new academic tools for visualization of plant cytokinesis, molecular cloning of onion mitotic genes, which are related to cell division, cell cycle, and chromosome segregation, is indispensable. Here we isolated CDC48 cDNA (AcCDC48) from onion seedlings, which encodes an AAA-ATPase chaperone and is known to play a role in cell division in broad species of organism. To characterize AcCDC48, we performed phylogenetic and DNA gel blot analyses, and discussed about the possible application of mammalian antibodies to detect it. The full-length onion CDC48 sequence provides useful information for future development of molecular markers to visualize cell division by using antibodies or GFP-fusion proteins.
著者
-
Suzuki Go
Division of Natural Science, Osaka Kyoiku University
-
Suzuki G
Div. Of Natural Sci. Osaka Kyoiku Univ.
-
Mukai Yasuhiko
Division Of Natural Sciences Department Of Arts And Sciences Osaka Kyoiku University
-
Shiomi Maho
Division of Natural Science, Osaka Kyoiku University
-
Morihana Sayuri
Division of Natural Science, Osaka Kyoiku University
関連論文
- Morphological and gene expression analysis under cool temperature conditions in rice anther development
- Novel self-compatible lines of Brassica rapa L. isolated from the Japanese bulk-populations
- Genomic organization of the AODEF gene in Asparagus officinalis L.
- Achievement of genetics in plant reproduction research: the past decade for the coming decade
- Identification of small RNAs in late developmental stage of rice anthers
- Comparative analysis of the S-intergenic region in class-II S haplotypes of self-incompatible Brassica rapa (syn. campestris)
- Genomic Organization of the S-Locus Region of Brassica(Biochemistry & Molecular Biology)
- Expression and developmental function of the 3-ketoacyl-ACP synthase2 gene in Arabidopsis thaliana
- Duplicated SP11 genes produce alternative transcripts in the S^ haplotype of Brassica oleracea
- Linear Dominance Relationship among Four Class-II S Haplotypes in Pollen is Determined by the Expression of SP11 in Brassica Self-Incompatibility
- SEQUENCE COMPARISON OF S-MULTIGENE FAMILY IN BRASSICA CAMPESTRIS
- Molecular Biology of Self-incompatibility in Brassica Species
- Expression of SLG^9 and SRK^9 Genes in Transgenic Tobacco
- Visualization of multiple T-DNA loci by FISH on extended DNA fibers
- Molecular Characterization of a 313-kb Genomic Region Containing the Self-incompatibility Locus of Ipomoea trifida, a Diploid Relative of Sweet Potato
- Efficient Storage and Screening System for Onion BAC Clones
- BAC FISH analysis in Allium cepa
- Physical Arrangement of Retrotransposon-Related Repeats in Centromeric Regions of Wheat
- Identification and Chromosomal Location of Tandemly Repeated DNA Sequences in Allium cepa
- Separated Transcriptomes of Male Gametophyte and Tapetum in Rice : Validity of a Laser Microdissection (LM) Microarray
- Various Spatiotemporal Expression Profiles of Anther-Expressed Genes in Rice
- Characterization of CDC48 in Allium cepa
- EXPRESSION OF SLG^9 AND SRK^9 GENOMIC CLONES IN TRANSGENIC TOBACCO
- UDP-Glucose Pyrophosphorylase is Rate Limiting in Vegetative and Reproductive Phases in Arabidopsis thaliana
- DNA methylation and histone modification in onion chromosomes
- Characterization of CDC48 in Allium cepa
- Molecular Cloning of Members of the S-Multigene Family in Self-Incompatible Brassica campestris L. : GENE STRUCTURE AND EXPRESSION : CELL-CELL INTERACTION
- Achievement of genetics in plant reproduction research : the past decade for the coming decade
- Morphological and gene expression analysis under cool temperature conditions in rice anther development
- Novel self-compatible lines of Brassica raga L. isolated from the Japanese bulk-populations
- DNA methylation and histone modification in onion chromosomes
- WS1-1 Large chromosomes and small chromosomes : Diversity of chromosome size(Workshop 1: "From large chromosomes to small chromosomes: Wide varieties of the genome and chromosome sizes",Abstracts of the Joint Meeting: the 58th Annual Meeting of the Societ
- Localization of transgene-derived friabilins in rice endosperm cells