Changing cell aggregations and lignification in tobacco suspension cultures

概要

論文の詳細を見る
The formation and dissociation of cell aggregates in tobacco cell suspensions were analyzed during 2,4-dichlorophenoxyacetic acid (2,4-D) (10^<-6> M) stock culture and during 6-benzyladenine (BA) (10^<-6> M) culture, for their effects on lignification. Aggregates were fractionated according to size by sieving them. Each fraction was cultured in both media and changes in the distribution of cells in the cluster fractions were followed during culture, after which the formation and dissociation rates of cells in aggregates were estimated. There was a significant difference in the formation and dissociation of cell aggregates between the 2,4-D culture and the BA culture. In the 2,4-D culture, aggregates dissociated to smaller ones or to single cells in the early growth stage, and then grew into large aggregates. Moreover, most cells had a uniform capacity for aggregate formation. In the BA culture, aggregates grew rapidly to a large size in the early growth stage. Lignification and tracheary element formation in the BA culture were especially stimulated in large aggregates derived from the large aggregates of the 2,4-D culture. Results of successive cultures of a particular cell aggregate fraction indicated that most cells had the potential to lignify or to differentiate tracheary elements. Thus, the switch-on of lignification or tracheid formation in the BA culture was determined only by cells in aggregate.
日本植物生理学会の論文