Effects of the Medium Type, Serum Amount and Dexamethasone Induction on the Cell Proliferation and Alkaline Phosphatase Activity of Twice-passaged SD Rats' Bone Marrow Stromal Cells

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The purpose of this investigation was to examine the effects of medium type (α-MEM or DMEM); serum amount (5, 10 or 15%); and dexamethasone (Dex) induction (Dex (-) or Dex (+)) on the proliferation and alkaline phosphatase (ALP) activity of twice-passaged SD rat's bone marrow stromal cells. (1) After 6 days culture (before confluence), cell number and total protein concentration were measured. It was found that the serum amount played the dominant role in increasing the proliferation. (2) After 24 days culture (long after confluence), total protein concentration and ALP activity were measured. It was found that α-MEM was superior to DMEM in producing larger total protein concentrations. Incrementing the serum amount tended to the increase in total protein concentrations. Dex induction (Dex(+)) decreased values of ALP activity / total protein concentration, reflecting the advance of the osteogenic differentiation stage. The use of Dex (+) α-MEM medium was preferred to produce greater protein production with higher osteogenic differentiation level
日本再生歯科医学会の論文
2004-03-30