Metabolism in vitro of 4-14C-Testosterone by Hepatic Microsomal and Soluble Fractions of Carbon Tetrachloride and Ethionine Injured Rats

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4-14C-testosterone was incubated with hepatic microsomal and soluble fractions of normal, CCl4 and ethionine administered male rats in the presence of NADPH at 37° for 90minutes. The metabolites produced were detected by thin layer autoradiography and chemical transformation. In the microsomal metabolism by the CCl4-injured liver, a high amount of the unmetabolized substrate remained and hydroxytestosterones were remarkably lower than those by the normal liver. These findings indicate that hydroxylation of testosterone is impaired by the administration of CCl4. In the soluble fraction, most of the substrate was not metabolized by the CCl4+Injured liver and little hydroxytestosterone was found. The amount of 5β-androstane-3β, 17β-diol, which was the predominant metabolite by the normal liver, was significantly reduced. These findings indicate the impairment of Δ4-5β-hydrogenase. In the microsomal metabolism by the ethionine-injured liver, the amount of the unmetabolized substrate was less than that in the microsomal metabolism by the CCl4-injured liver, but the hydroxylation of testosterone was not significantly suppressed. In the metabolism by the soluble fraction, a slightly higher amount of the unmetabolized substrate was found, but no impairment of Δ4-5β-hydrogenase was observed.
社団法人日本内分泌学会の論文