Gene Expression Analyses of Human Mesenchymal Stem Cells Cultured in Osteogenic Differentiation Medium for 3, 7, 14 and 21 Days by Genome Focus DNA Microarray and Real-time PCR
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概要
- 論文の詳細を見る
The purpose of this study was to evaluate gene expressions of human mesenchymal stem cells (hMSC) cultured in osteogenic differentiation medium (OM) which contained ascorbic acid, β-glycerophosphate and dexamethasone for 0 (control), 3, 5, 7, 14 and 21 days by 8.5k Genome Focus DNA microarray and real-time PCR. It was confirmed by the DNA microarray analysis that 327 genes of hMSC were significantly up-regulated by culture in OM especially at 14 and 21 days while 156 genes were down-regulated. Up-regulated genes included osteoblast-related genes such as secreted phosphoprotein 1 (osteopontin) gene and hypothetical protein expressed in osteoblast gene, along with angiogenesis-related genes and cell cycle arrest-related genes, while down-regulated genes contained stroma-related genes and keratin-related genes. Expressions of several osteogenic differentiation marker genes such as (down-regulated) osteonectin gene and (up-regulated) BMP2 gene were also evaluated by real-time PCR. Gene expression database identified here might contribute to dental tissue engineering.
- 日本再生歯科医学会の論文
- 2007-09-30
著者
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TAIRA Masayuki
Department of Dental Materials and Technology, Iwate Medical University School of Dentistry
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成島 尚之
Department Of Materials Processing Tohoku University
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Sasaki Kaori
Department of Integrated Medicine and Informatics, Ehime University Graduate School of Medicine
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Sasaki Kaori
Division Of Dental Bio-engineering Department Of Pathogenesis And Control Of Oral Diseases Iwate Med
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NEZU Takashi
Department of Prosthetic Dentistry I, Faculty of Dentistry, Kyushu University
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Taira Masayuki
Division Of Dental Bio-engineering Department Of Pathogenesis And Control Of Oral Diseases Iwate Med
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Saitoh Setsuo
Division Of Dental Bio-engineering Department Of Pathogenesis And Control Of Oral Diseases Iwate Med
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SAITOH Setsuo
Department of Dental Materials Science and Technology, Iwate Medical University School of Dentistry
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ARAKI Yoshima
Department of Dental Materials Science and Technology, Iwate Medical University School of Dentistry
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CHOSA Naoyuki
Department of Biochemistry, Iwate Medical University School of Dentistry
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SATO Nobuko
Department of Biochemistry, Iwate Medical University School of Dentistry
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Sasaki Kaori
Department Of Applied Chemistry Faculty Of Science And Engineering Kinki University
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Nezu Takashi
Department Of Dental Materials Science And Technology Iwate Medical University School Of Dentistry
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Sato Nobuko
Department Of Biochemistry Iwate Medical University School Of Dentistry
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Chosa Naoyuki
Department Of Biochemistry Iwate Medical University School Of Dentistry
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Araki Y
Department Of Dental Materials Science And Technology Iwate Medical University School Of Dentistry
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Araki Yoshima
Department Of Dental Materials Science And Technology Iwate Medical University School Of Dentistry
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Nezu Takashi
Department Of Dental Biomaterials And Technology Iwate Medical University School Of Dentistry
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Nezu Takashi
Department of Chemistry, Faculty of Science, Nagoya University
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TAIRA Masayuki
Department of Biomedical Engineering, Iwate Medical University,
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