Proteome analyses of human macrophages exposed to low cytotoxic IC90 copper (2+) ions
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概要
- 論文の詳細を見る
Dental noble alloys often contain copper (Cu). Eluted metal ions sometimes irritate oral tissues. The most eluted ions are Cu ions. The purpose of this study was to investigate the effects of low cytotoxic (IC90, 100 μmol/L) Cu ions on macrophages by proteome analyses consisting of two-dimensional (2D) electrophoresis and matrix-assisted laser desorption/ionization —time of flight (MALDI-TOF) mass spectrometry. The analyses revealed that stimulation with IC90 Cu ions for 1 day caused the macrophage to significantly increase five specific protein spots. Mascot peptide mass finger-print matching suggested that four of them were attributed to 70 kDa heat shock protein 1A/1B (HSP70). HSP70 expression was verified by expressions of corresponding HSPA1A and HSPA1B mRNAs of the macrophage in quantitative real-time PCR analyses. It was concluded that by producing abundant HSP70, the macrophage protected itself against intracellularly intruding cytotoxic Cu ions that might un-fold and crosslink cellular proteins.
- 2011-05-01
著者
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成島 尚之
Department Of Materials Processing Tohoku University
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Nezu Takashi
Division Of Dental Bio-engineering Department Of Pathogenesis And Control Of Oral Diseases Iwate Med
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Taira Masayuki
Division Of Dental Bio-engineering Department Of Pathogenesis And Control Of Oral Diseases Iwate Med
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Nezu Takashi
Department Of Dental Materials Science And Technology Iwate Medical University School Of Dentistry
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Harada Hidemitsu
Division Of Oral Histology And Developmental Biology Department Of Function And Structure School Of
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Kagiya Tadayoshi
Department Of Oral Anayomy Ii Iwate Medical University School Of Dentistry
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Kagiya Tadayoshi
Division Of Oral Anatomy Department Of Oral Biology Iwate Medical University School Of Dentistry
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Sasaki Minoru
Division Of Oral Microbiology And Immunology Department Of Pathogenesis And Control Of Oral Diseases
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SHIMOYAMA Yu
Division of Oral Microbiology and Immunology, Department of Pathogenesis and Control of Oral Disease
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KIMURA Shigenobu
Division of Oral Microbiology and Immunology, Department of Pathogenesis and Control of Oral Disease
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Harada Hidemitsu
Division Of Oral Histology And Developmental Biology Department Of Oral Structure And Function Biolo
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Kimura Shigenobu
Division Of Oral Microbiology And Immunology Department Of Pathogenesis And Control Of Oral Diseases
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Shimoyama Yu
Division Of Oral Microbiology And Immunology Department Of Pathogenesis And Control Of Oral Diseases
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KIMURA Shigenobu
Division of Molecular Microbiology, Department of Microbiology, Iwate Medical University, Yahaba-chou, Iwate, Japan.
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