RECEPTOR-MEDIATED BINDING OF ELASTIN-DERIVED HEXAPEPTIDE TO MACROPHAGES INDUCED A GALACTOSUGAR-SENSITIVE CHEMOTAXIS

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概要

• 論文の詳細を見る

• Elastin, a highly hydrophobic protein, is a predominant component of mature elastic fibers which confers elasticity and resiliency to the vascular walls, lungs, skin and ligaments. Elastin digests and elastin-derived peptides interact with a variety of cell types to modulate cellular behavior and are implicated to play roles in several diseases such as atherosclerosis, emphysema and metastasis. Our previous study showed polyhexapeptide (VGVAPG)n as a chemoattractant. The circular permutations of VGVAPG were investigated for their chemotactic ability. It was observed that four of the six permutations namely: VGVAPG, GVAPGV, VAPGVG and GVGVAP, were chemoattractant for macrophages and that they serve as signaling peptides which function to regulate macrophage migration. Chemotactic activity induced by these peptides was found to be profoundly sensitive to lactose. Moreover, deactivation study indicated recognition of these four hexapeptides by a single receptor. Macrophages stimulated by VGVAPG demonstrated an enhancement in guanosine 3', 5'-cyclic monophosphate (cGMP) level, thus, reflecting an involvement of cGMP in the signaling mechanism of VGVAPG-induced chemotaxis. However, cGMP elevation was found to be lactose sensitive. It is hypothesized that chemotaxis in response to these elastin peptides is mediated by the action of cell surface receptor. With this working hypothesis. we investigated binding of VGVAPG to macrophages. To facilitate radiolabeling of the peptide for the receptor binding studies, VGVAPG was synthesized with a tyrosine residue to yield Y-VGVAPG. Our report will provide evidence for an elastin-derived peptide receptor present on the cell surface of macrophages.

• 日本結合組織学会の論文

著者

• Takami N

  Department Of Dental Materials Science And Technology Iwate Medical University School Of Dentistry

• 岡本 研

  日本医科大学大学院医科生物化学分野

• 岡本 研

  日本医科大学生化学分子生物学講座(構造生物学・代謝学)

• MAEDA Iori

  Department of Chemistry, Faculty of Science, Kyushu University

• Maeda I

  Department Of Bioscience And Bioinformatics Kyushu Institute Of Technology

• Maeda Iori

  Department Of Biochemical Engineering And Science Kyushu Institute Of Technology

• Takeru Nose

  Department Of Chemistry Faculty Of Sciences Kyushu University

• Nezu Takashi

  Division Of Dental Bio-engineering Department Of Pathogenesis And Control Of Oral Diseases Iwate Med

• Briones Maria

  Department Of Biochemical Engineering And Science Kyushu Institute Of Technology

• Briones Maria

  九州工業大学情報工学部生物化学システム工学科

• OKAMOTO Kouji

  Department of Biochemical Engineering and Science, Kyushu Institute of Technology

• Okamoto Kouji

  Department Of Biochemical Engineering And Science Kyushu Institute Of Technology

• Nose Takeru

  Laboratory Of Biochemistry Department Of Chemistry Faculty Of Science Kyushu University

• Takami Noboru

  RI Center, Fukuoka University School of Medicine

• Kamisato Satsuki

  Department of Biochemical Engineering and Science, Kyushu Institute of Technology

• Okamoto Ken-ichi

  Graduate School Of Pure And Applied Sciences University Of Tsukuba

• Kamisato Satsuki

  Department Of Biochemical Engineering And Science Kyushu Institute Of Technology

• Okamoto Ken-ichi

  Department Of Advanced Materials Science And Engineering Faculty Of Engineering Yamaguchi University

• Okamoto Kouji

  Department Of Biochemical Engineering And Science Kyusyu Institute Of Technology

• Okamoto Ken

  Department Of Biochemistry And Molecular Biology Nippon Medical School

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