Effect of HEMA Adsorption on the Stability of Native and Denatured Type I Tendon Collagen

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概要

• 論文の詳細を見る

• Adsorption behavior and stabilization/destabilization effects of 2-hydroxyethyl methacrylate (HEMA) on a bovine tendon collagen (BTC)(type I), either native (N) or thermally denatured (D), were studied by IR spectroscopy and differential scanning calorimetry (DSC). The amount of HEMA adsorbed was larger on BTC(D) than on BTC(N), because BTC(D) had a larger specific surface area (SSA) as revealed from SSA measurement. Denaturation temperature (T_d) of BTC(N) , measured by DSC in aqueous HEMA solution, decreased from 63℃ to 40℃ with increasing HEMA concentration (C_HEMA) up to 20wt%. This destabilization might be caused by the loss of hydrophobic stabilization of the helix structure as C_HEMA was increased. At C_HEMA>20wt%, the structure of collagen was restabilized presumably due to the dehydration effect conferred by HEMA at higher concentration. BTC(D) with little helix content, however, showed only a weak endothermic peak in the DSC measurement and the T_d at 40℃ was independent of C_HEMA.

• 日本歯科理工学会の論文

著者

• Takami N

  Department Of Dental Materials Science And Technology Iwate Medical University School Of Dentistry

• 成島 尚之

  Department Of Materials Processing Tohoku University

• NEZU Takashi

  Department of Prosthetic Dentistry I, Faculty of Dentistry, Kyushu University

• Nezu Takashi

  Division Of Dental Bio-engineering Department Of Pathogenesis And Control Of Oral Diseases Iwate Med

• Nezu T

  Department Of Dental Biomaterials And Technology Iwate Medical University School Of Dentistry

• TERADA Yoshihiro

  Section of Fixed Prosthodontics, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu

• Nezu Takashi

  Department Of Dental Materials Science And Technology Iwate Medical University School Of Dentistry

• Terada Y

  Fixed Prosthodontics Division Of Oral Rehabilitation Faculty Of Dental Science Kyushu University

• MORIKAWA Tomohiro

  Section of Fixed Prosthodontics, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu

• FUKUDA Kyosuke

  Section of Fixed Prosthodontics, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu

• Morikawa Tomohiro

  Fixed Prosthodontics Division Of Oral Rehabilitation Faculty Of Dental Science Kyushu University

• Fukuda Kyosuke

  Section Of Fixed Prosthodontics Division Of Oral Rehabilitation Faculty Of Dental Science Kyushu Uni

• Terada Yoshihiro

  Section Of Fixed Prosthodontics Division Of Oral Rehabilitation Faculty Of Dental Science Kyushu Uni

• Nezu Takashi

  Department Of Dental Biomaterials And Technology Iwate Medical University School Of Dentistry

• Nezu Takashi

  Department of Chemistry, Faculty of Science, Nagoya University

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