Accumulation of Element Ti in Macrophage-like RAW264 Cells Cultured in Medium with 1 ppm Ti and Effects on Cell Viability, SOD Production and TNF-α Secretion
スポンサーリンク
概要
- 論文の詳細を見る
The adverse effect of Ti on body-defense macrophage is not well understood. The aims of this study were twofold: (1) to examine the intracellular accumulation of Ti element; and (2) to measure the cell viability, superoxide dismutase (SOD) production, and TNF-α secretion of macrophage-like RAW264 cells cultured for two days in medium with 1 ppm Ti prepared from acidic ICP Ti standard solution. PIXE analysis showed that element Ti was accumulated up to 7.3 ppm in RAW264 cells when cultured in the medium with 1 ppm Ti. Further, RAW264 cells cultured in the medium with 1 ppm Ti exhibited cell viability of about 60%, SOD production of about 180%, and TNF-α secretion of about 170% relative to those of control cells cultured in the medium without Ti. It was speculated that phagocytosis of minute Ti-containing complex (mostly TiO_2) by macrophage caused oxidative stress and inflammatory reaction, leading to cell proliferation arrest and increased production of SOD and TNF-α.
- 日本歯科理工学会の論文
著者
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SASAKI Minoru
Dept. of Advanced Science and Technology, Toyota Technological Institute
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NARUSHIMA Takayuki
Tohoku University Biomedical Engineering Research Organization, Tohoku University
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成島 尚之
Department Of Materials Processing Tohoku University
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Sasaki Kaori
Division Of Dental Bio-engineering Department Of Pathogenesis And Control Of Oral Diseases Iwate Med
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Taira Masayuki
Division Of Dental Bio-engineering Department Of Pathogenesis And Control Of Oral Diseases Iwate Med
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Saitoh Setsuo
Division Of Dental Bio-engineering Department Of Pathogenesis And Control Of Oral Diseases Iwate Med
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Narushima Takayuki
Tohoku University Biomedical Engineering Research Organization (tubero) Aobayama Materials Science B
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SERA Kouichiro
Cyclotron Research Center, Iwate Medical University
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ARAKI Yoshima
Department of Dental Materials Science and Technology, Iwate Medical University School of Dentistry
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TAIRA Masayuki
Dept. of Dental Materials Science and Technology, Iwate Medical University School of Dentistry
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SASAKI Kaori
Dept. of Dental Materials Science and Technology, Iwate Medical University School of Dentistry
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SAITOH Setsuo
Dept. of Dental Materials Science and Technology, Iwate Medical University School of Dentistry
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NEZU Takashi
Dept. of Dental Materials Science and Technology, Iwate Medical University School of Dentistry
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KIMURA Shigenobu
Dept. of Oral Microbiology, Iwate Medical University School of Dentistry
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TERASAKI Kazunori
Cyclotron Research Center, Iwate Medical University
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ARAKI Yoshima
Dept. of Dental Materials Science and Technology, Iwate Medical University School of Dentistry
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Araki Y
Department Of Dental Materials Science And Technology Iwate Medical University School Of Dentistry
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Sera Kouichiro
Cyclotron Research Center Iwate Medical University
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Sasaki Minoru
Division Of Oral Microbiology And Immunology Department Of Pathogenesis And Control Of Oral Diseases
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Kimura Shigenobu
Division Of Oral Microbiology And Immunology Department Of Pathogenesis And Control Of Oral Diseases
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Sasaki Minoru
Dept. Of Advanced Science And Technology Toyota Technological Institute
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Terasaki Kazunori
Cyclotron Research Center Iwate Medical University
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Sasaki Minoru
Dept. Of Advanced Sci. And Technol. Toyota Technological Inst.
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