TEM/EDX Observation of Micro-beads of Magnetically Activated Cell Sorting (MACS) System and Separation Trial of Mouse Mesenchymal Stem Cells from Bone Marrow Cells by MACS System
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概要
- 論文の詳細を見る
Magnetically activated cell sorting (MACS) system is one isolation technique of mesenchymal stem cells (MSC) from bone marrow cells. TEM/EDX observation revealed that its micro-beads had the size of about 0.5 to 1.0μm and consisted of matrix and inorganic phases. The inorganic phase contained small particles of about 40nm size, and consisted of Fe, Si, O, Cl (e.g., Fe_3O_4+SiO_2+FeCl_3). In MACS system, lineage (hematopoietic) cell depletion kit reduced the cell number of bone marrow cells to 1.2%. Subsequent positive selection by CD117, anti-Sca-1, CD34 and CD44 micro-beads lead to the cell collection rates of 0.57%, 0.10%, 0.13% and 0.14%, respectively. A combination of three micro-beads for lineage cell depletion kit, CD117 and anti-Sca-1 micro-beads succeeded in reducing the cell collection rate down to 0.04%, matching a normal existing rate of MSC in bone marrow cells (0.01 to 0.10%).
- 日本再生歯科医学会の論文
- 2008-09-30
著者
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TAIRA Masayuki
Department of Dental Materials and Technology, Iwate Medical University School of Dentistry
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成島 尚之
Department Of Materials Processing Tohoku University
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Sasaki Kaori
Department of Integrated Medicine and Informatics, Ehime University Graduate School of Medicine
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HIRATA Isao
Department of Biomaterials Science, Graduate School of Biomedical Sciences, Hiroshima University
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Sasaki Kaori
Division Of Dental Bio-engineering Department Of Pathogenesis And Control Of Oral Diseases Iwate Med
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Sasaki Minoru
Department of Acoustic Design,Kyushu Institute of Design
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HARADA Hidemitsu
Department of Oral Anatomy and Cell Biology, Kyushu Dental College
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NEZU Takashi
Department of Prosthetic Dentistry I, Faculty of Dentistry, Kyushu University
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Taira Masayuki
Division Of Dental Bio-engineering Department Of Pathogenesis And Control Of Oral Diseases Iwate Med
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Saitoh Setsuo
Division Of Dental Bio-engineering Department Of Pathogenesis And Control Of Oral Diseases Iwate Med
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SAITOH Setsuo
Department of Dental Materials Science and Technology, Iwate Medical University School of Dentistry
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ARAKI Yoshima
Department of Dental Materials Science and Technology, Iwate Medical University School of Dentistry
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KAGIYA Tadayoshi
Department of Oral Anayomy II, Iwate Medical University School of Dentistry
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KIMURA Shigenobu
Department of Oral Microbiology, Iwate Medical University School of Dentistry
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Sasaki Kaori
Department Of Applied Chemistry Faculty Of Science And Engineering Kinki University
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Nezu Takashi
Department Of Dental Materials Science And Technology Iwate Medical University School Of Dentistry
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Harada Hidemitsu
Department Of Anatomy And Developmental Biology Osaka University Graduate School Of Dentistry
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Hirata Isao
Department Of Biomaterials Science Graduate School Of Biomaterials Science Hiroshima University
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Araki Y
Department Of Dental Materials Science And Technology Iwate Medical University School Of Dentistry
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Araki Yoshima
Department Of Dental Materials Science And Technology Iwate Medical University School Of Dentistry
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Hirata Isao
Department Of Biomaterial Science Division Of Molecular Medical Science Hiroshima University Graduat
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Kimura Shigenobu
Department Of Biology Faculty Of Science Kumamoto University
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Kagiya Tadayoshi
Department Of Oral Anayomy Ii Iwate Medical University School Of Dentistry
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Sasaki Minoru
Division Of Oral Microbiology And Immunology Department Of Pathogenesis And Control Of Oral Diseases
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Harada Hidemitsu
Division Of Oral Histology And Developmental Biology Department Of Oral Structure And Function Biolo
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Kimura Shigenobu
Division Of Oral Microbiology And Immunology Department Of Pathogenesis And Control Of Oral Diseases
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Sasaki Minoru
Department Of Acoustic Design Kyushu Institute Of Design
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Nezu Takashi
Department Of Dental Biomaterials And Technology Iwate Medical University School Of Dentistry
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Nezu Takashi
Department of Chemistry, Faculty of Science, Nagoya University
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TAIRA Masayuki
Department of Biomedical Engineering, Iwate Medical University,
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