Apoptotic and Necrotic Influence of Dental Resin Polymerization Initiators in Human Gingival Fibroblast Cultures

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概要

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• The aim of this study was to examine the apoptotic and necrotic influence of four dental resin polymerization initiators-namely benzoyl peroxide (BPO), camphorquinone (CQ), dimethylaminoethyl methacrylate (DMAEMA), and dimethyl-para-toluidine (DMPT)-on human gingival fibroblast (HGF) cells. To this end, the growth inhibition of HGF cells with 1 mM BPO, CQ, and DMAEMA, and 500 μM DMPT was evaluated using Cell Counting Kit-8. Then, cell cycle analysis by flow cytometry was used to assess propidium iodide-stained cells (distribution of cells in G_0/G_1, S, G_2/M phases). All four dental resin polymerization initiators induced G_0/G_1 cell cycle arrest. As for the patterns of cell death (necrosis and/or apoptosis), they were analyzed using Annexin V-FITC/PI staining with flow cytometry. All four dental resin polymerization initiators most likely induced necrosis.

著者

• NOMURA Yuji

  Department of Biomaterials Science, Graduate School of Biomedical Science, Hiroshima University

• BHAWAL Ujjal

  Department of Oral and Maxillofacial Surgery & High-Tech Research Center, Kanagawa Dental College

• OKAZAKI Masayuki

  Department of Biomaterials Science, Graduate School of Biomedical Science, Hiroshima University

• HIRATA Isao

  Department of Biomaterials Science, Graduate School of Biomedical Sciences, Hiroshima University

• Nomura Yuji

  Hiroshima Univ. Hiroshima Jpn

• Nomura Yuji

  Department Of Biomaterials Science Graduate School Of Biomedical Science Hiroshima University

• Okazaki Masayuki

  Department Of Fixed Prosthodontics Osaka University Graduate School Of Dentistry

• Okazaki Masayuki

  Department Of Biomaterials Science Graduate School Of Biomedical Science Hiroshima University

• Sawajiri Masahiko

  Department Of Oral And Maxillofacial Radiology Graduate School Of Biomedical Science Hiroshima Unive

• Hirata Isao

  Hiroshima Univ. Hiroshima Jpn

• Bhawal Ujjal

  Department Of Oral And Maxillofacial Surgery & High-tech Research Center Kanagawa Dental College

• Bhawal Ujjal

  Department Of Molecular Pathology Nara Medical University School Of Medicine

• MASUKI Kouhei

  Department of Biomaterials Science, Graduate School of Biomedical Science, Hiroshima University

• SAWAJIRI Masahiko

  Hiroshima University Hospital

• NAHARA Yukinori

  Yamasaki General Hospital

• Masuki Kouhei

  Department Of Biomaterials Science Graduate School Of Biomedical Science Hiroshima University

• Hirata Isao

  Department Of Biomaterial Science Division Of Molecular Medical Science Hiroshima University Graduat

• Okazaki Masayuki

  Hiroshima Univ. Hiroshima Jpn

• Okazaki Masayuki

  Department Of Biomaterial Science Division Of Molecular Medical Science Hiroshima University Graduat

• Bhawal Ujjal

  Department Of Molecular Pathology Nara Medical University

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