Effects of high-dose major components in oral disinfectants on the cell cycle and apoptosis in primary human gingival fibroblasts in vitro
スポンサーリンク
概要
- 論文の詳細を見る
We evaluated the effects of high-dose major components in oral disinfectants on oral cells from the standpoints of the cell cycle and apoptosis. We examined the viability and cell cycle of human gingival fibroblasts (HGFs) treated with the components of dental disinfectants, benzethonium chloride (BEC), benzalkonium chloride (BAC), and povidone iodine (PVD-I) using a cell counting kit and flow cytometry. The IC50 inhibitory concentration value in HGF cultures at 24 hours was 1.3×10–2 mM BEC, 6.0×10–3 mM BAC, and 2.6×10–1 mM PVD-I. In the cell cycle analysis, propidium iodide-stained HGFs were arrested in G0/G1 of the cell cycle by all three disinfectants, and in the apoptosis assay, annexin V-FITC/PI-stained HGFs that became apoptotic at 5.0×10–2 and 1.0×10–1 mM BEC and 5.0×10–2 and 1.0×10–1 mM BAC, but not in PVD-I at concentrations as high as 5.0×10–1 mM. Our findings describe the effects of high-dose oral disinfectants, rather than clinical concentrations. Nevertheless, appreciating the effects of high-dose disinfectants absorbed into the human body is important, where they may accumulate in specific tissues and cells.
- 2010-01-01
著者
-
NOMURA Yuji
Department of Biomaterials Science, Graduate School of Biomedical Science, Hiroshima University
-
BHAWAL Ujjal
Department of Oral and Maxillofacial Surgery & High-Tech Research Center, Kanagawa Dental College
-
NISHIKIORI Ryo
Department of Biomaterials Science, Graduate School of Biomedical Science, Hiroshima University
-
SAWAJIRI Masahiko
Department of Oral and Maxillofacial Radiology, Graduate School of Biomedical Science, Hiroshima Uni
-
MAEDA Takeshi
Department of Prothetic Dentistry, Graduate School of Biomedical Science, Hiroshima University
-
OKAZAKI Masayuki
Department of Biomaterials Science, Graduate School of Biomedical Science, Hiroshima University
-
Nomura Yuji
Hiroshima Univ. Hiroshima Jpn
-
Nomura Yuji
Department Of Biomaterials Science Graduate School Of Biomedical Science Hiroshima University
-
Nishikiori Ryo
Department Of Biomaterials Science Graduate School Of Biomedical Science Hiroshima University
-
Maeda Takeshi
Departmant Of Orthopaedic Surgery Kyushu University
-
Okazaki Masayuki
Department Of Fixed Prosthodontics Osaka University Graduate School Of Dentistry
-
Okazaki Masayuki
Department Of Biomaterials Science Graduate School Of Biomedical Science Hiroshima University
-
Sawajiri Masahiko
Department Of Oral And Maxillofacial Radiology Graduate School Of Biomedical Science Hiroshima Unive
-
Bhawal Ujjal
Department Of Oral And Maxillofacial Surgery & High-tech Research Center Kanagawa Dental College
-
Bhawal Ujjal
Department Of Molecular Pathology Nara Medical University School Of Medicine
-
Okazaki Masayuki
Hiroshima Univ. Hiroshima Jpn
-
Okazaki Masayuki
Department Of Biomaterial Science Division Of Molecular Medical Science Hiroshima University Graduat
-
Maeda Takeshi
Department Of Prothetic Dentistry Graduate School Of Biomedical Science Hiroshima University
-
Bhawal Ujjal
Department Of Molecular Pathology Nara Medical University
関連論文
- Effects of high-dose major components in oral disinfectants on the cell cycle and apoptosis in primary human gingival fibroblasts in vitro
- <Symposium I>Vascular endothelial growth factor( VEGF) expression is negatively regulated by basic-helix-loop-helix( bHLH)transcription factor DEC2
- Creation of highly functional CO_3Ap-collagen scaffold biomaterials
- Apoptotic and Necrotic Influence of Dental Resin Polymerization Initiators in Human Gingival Fibroblast Cultures
- Chemical analyses of hydroxyapatite formation on SAM surfaces modified with COOH, NH_2, CH_3, and OH functions
- Analysis of Chemical Interaction of 4-MET with Hydroxyapatite Using XPS
- Spectroscopic Characterization of Enamel Surfaces Irradiated with Er:YAG Laser
- Effect of Presilanization Filler Decontamination on Aesthetics and Degradation Resistance of Resin Composites
- An Unusually Large Submandibular Salivary Calculus: Case Report and Structural Analysis
- Chemical analyses of hydroxyapatite formation on SAM surfaces modified with COOH, NH_2, CH_3, and OH functions
- Standardized Multicolored Magnetic Resonance Images of Gynecologic Lesions
- The Synergistic Effect of Bactobolamine and Tacrolimus on In Vitro and In Vivo Experiments
- A Nonspecific Colonic Ulcer Occurring After Hepatectomy: Report of a Case
- Synthesis and Structure of Optically Active Polyesters Containing C_2 Chiral Spirobifluorene Moieties in the Main Chain
- Synthetic Osteopontin-derived Peptide SVVYGLR can Induce Neovascularization in Artificial Bone Marrow Scaffold Biomaterials
- Role of Acidic Amino Acid for Regulating Hydroxyapatite Crystal Growth
- Advantages of TOF-SIMS Analysis of Hydroxyapatite and Fluorapatite in Comparison with XRD, HR-TEM and FT-IR
- Osteopontin-derived Peptide SVVYGLR Induces Angiogenesis In Vivo
- Atlantoaxial Instability in Neck Retraction and Protrusion Positions in Patients With Rheumatoid Arthritis
- Progression of joint destruction in patients with rheumatoid arthritis are related to in vitro bone resorption with synovial cells
- New Type Scaffold Biomaterials with Magnesium Accelerating Osteoblast Adhesion and Bone Formation
- Morphological and Crystallographic Properties of Tooth Enameloid of Medaka (Oryzias latipes)
- Multicentric reticulohistiocytosis with hydrarthrosis in both knee joints: disease stabilization with synovectomy, and medication with a steroid and low-dose methotrexate
- Menopausal syndrome in female patients with rheumatoid arthritis
- Effect of PMMA polymer on the dynamic viscoelasticity and plasticizer leachability of PEMA-based tissue conditioners
- Influence of Storage Methods on the Surface Roughness of Tissue Conditioners
- High mobility group box-1-inducible melanoma inhibitory activity is associated with nodal metastasis and lymphangiogenesis in oral squamous cell carcinoma
- Degree of immobilization of synthetic RGDS(PO_3H_2)PA peptides on titanium surfaces
- A Rational Design of Helix: Absolute Helix Synthesis by Binaphthyl-Salen Fusion
- SEM Observation of Collagen Fibrils Secreted from the Body Surface of Osteoblasts on a CO_3apatite-collagen Sponge
- The Occurrence of Dual-gender Imposex in Japanese Freshwater Crab
- Bone formation ability of carbonate apatite-collagen scaffolds with different carbonate contents
- Estrogenic Activity of Phthalate Esters by In Vitro VTG Assay Using Primary-cultured Xenopus Hepatocytes
- Change in Microstructure and Solubility Improvement of HAp Ceramics by Heat-Treatment in a Vacuum
- Long Adult Spinal Deformity Fusion to the Sacrum Using rhBMP-2 Versus Autogenous Iliac Crest Bone Graft
- Osteoblast Proliferation Behavior and Bone Formation on and in CO_3apatite-Collagen Sponges with a Porous Hydroxyapatite Frame
- Does Ossification of the Posterior Longitudinal Ligament Affect the Neurological Outcome After Traumatic Cervical Cord Injury?
- Studies on Laser Applications to Dental Hard Tissues
- Development of Biomedical Materials Carrying Immobilized Functional Peptides and Discovery of an Angiogenic Peptide
- Detailed Consideration of Physicochemical Properties of CO_3apatites as Biomaterials in Relation to Carbonate Content Using ICP, X-ray Diffraction, FT-IR, SEM, and HR-TEM
- Monitoring masseter muscle evoked responses enables faster tracheal intubation
- Acceleration of bone formation with BMP2 in frame-reinforced carbonate apatite-collagen sponge scaffolds
- The Spectrum of Schrodinger operators with periodic Aharonov-Bohm magnetic fields (Spectral and Scattering Theory and Related Topics)
- Heavy Metal Contamination in the Sediment and its Effect on the Occurrence of the Most Dominant Bivalve, Ruditapes philippinarum, on the Tidal Flats of Ariake Bay in Kumamoto Prefecture, the West Coast of Kyushu, Japan
- Immobilization of Simulated Reducing Agent at the Surface of SiO_2 Fillers in Dental Composite Resins
- A Novel Technique for Surgical Resection of Spinal Meningioma
- Cell Adhesion and Proliferation Patterns on Mixed Self-assembled Monolayers Carrying Various Ratios of Hydroxyl and Methyl Groups
- Higher Concentrations of Fluoride Ions Dramatically Inhibit the Survival of Osteoblasts
- Building environmental models of man-made environments by panoramic sensing
- Development of Biomimetic Materials : Novel Composite Material Carrying Immobilized Functional Peptides
- Downregulation of runt-related transcription factor 3 associated with poor prognosis of adenoid cystic and mucoepidermoid carcinomas of the salivary gland
- The dynamic viscoelasticity and water absorption characteristics of soft acrylic resin materials containing adipates and a maleate plasticizer
- PRE-SYNAPTIC RELEASE OF EXCITATORY AMINO ACID IN CONTUSED BRAIN TISSUE: EFFECTS OF HYPOTHERMIA AND IN SITU ADMINISTRATION OF Co^ ON EXTRACELLULAR LEVELS OF GLUTAMATE
- Analysis of the Risk Factors for Severity of Neurologic Status in 216 Patients With Thoracolumbar and Lumbar Burst Fractures
- Drug-induced Interstitial Pneumonitis due to Low-dose Lenalidomide
- A novel glass ionomer cement containing MgCO_3 apatite induced the increased proliferation and differentiation of human pulp cells in vitro
- Influence of air-abrasion and subsequent heat treatment on bonding between zirconia framework material and indirect composites
- Facilitated transport of CO2 through supported liquid membranes of various amine solutions-effects of rate and equilibrium of reaction between CO2 and amine-.
- A Large Calculus in the Parotid Duct
- Osteoma in Ethmoid Sinus: a case report