Detailed Consideration of Physicochemical Properties of CO_3apatites as Biomaterials in Relation to Carbonate Content Using ICP, X-ray Diffraction, FT-IR, SEM, and HR-TEM

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CO_3apatites with different carbonate contents were synthesized at 60±1℃) and pH 7.4±0.2 under different carbonate concentrations (0-0.3 mol/L) in the supplied solutions. Their physicochemical properties were analyzed using various methods. Inductively coupled plasma gave accurate chemical analysis data for calcium and phosphate contents. X-ray diffraction analysis showed a clear chemical shift at high carbonate content. A CO_3^<2-> absorption peak area approximately proportional to carbonate content was observed through Fourier transmission infrared spectroscopy. Scanning electron microscopy and high-resolution transmission electron microscopy revealed a dramatic change of the crystal shape. Osteoblast proliferation at the surface of each CO_3apatite-collagen sponge indicated that osteoblasts deformed to expand and cover the surface of the sponge, and appeared to adhere well to the sponge.
日本歯科理工学会の論文