New development of carbonate apatite-chitosan scaffold based on lyophilization technique for bone tissue engineering
スポンサーリンク
概要
- 論文の詳細を見る
Carbonate apatite-chitosan scaffolds (CA-ChSs) were fabricated using the lyophilization technique. It was found that ChSs prepared with 200 mg chitosan powder (ChSs200) had well-structured three-dimensional architecture with high porosity and good retentive form without brittleness. In addition, it was shown that the number of osteoblast-like cells MC3T3-E1 proliferated on desalinated ChSs200 was larger than that on the non-desalinated ChSs200. CA-ChSs were fabricated by adding 100 mg carbonate apatite (CA) to 200 mg chitosan gels followed by freeze-drying (CA100ChSs200). SEM observation revealed that CA100ChSs200 had favorable three- dimensional porous structures. The number of living cells increased more rapidly on CA100ChSs200 prepared with different amounts of CA than on ChSs. ALP activity significantly increased after day 14 and reached a plateau after day 21 in ChSs200 and CA100ChSs200. It was concluded that newly developed CA100ChSs200 may be a possible scaffold material for tissue engineering.
著者
-
Kubo Takayasu
Clinic Of Oral Implants Hiroshima University Hospital
-
Hirata Isao
Department Of Biomaterial Science Division Of Molecular Medical Science Hiroshima University Graduat
-
Kato Koichi
Department Of Biology Graduate School Of Natural Sciences Nagoya City University
-
Akagawa Yasumasa
Department Of Advanced Prosthodontics Division Of Cervico-gnathostomatology Graduate School Of Biome
-
AKAGAWA Yasumasa
Department of Advanced Prosthodontics, Hiroshima University Graduate School of Biomedical Sciences
-
Ariani Maretaningtias
Prosthodontics Department, Faculty of Dentistry, Airlangga University
-
MATSUURA Ayumu
Clinic of Oral Implants, Hiroshima University Hospital
関連論文
- Apoptotic and Necrotic Influence of Dental Resin Polymerization Initiators in Human Gingival Fibroblast Cultures
- Cyclic ADP-Ribose Mediates Formyl Methionyl Leucyl Phenylalanine (fMLP)-Induced Intracellular Ca2+ Rise and Migration of Human Neutrophils
- Chemical analyses of hydroxyapatite formation on SAM surfaces modified with COOH, NH_2, CH_3, and OH functions
- Dental status and mortality in institutionalized elderly people
- Dentin-derived Proteins Enhance Fibronectin-related but not Vitronectin-related Specific Adhesion of Human Periodontal Ligament Cells
- Mechanical Loading of Implants Prevents Resorption of Grafted Autogenous Bone in Maxilla : A Case Report of 3-year Clinical Follow-up
- Primary Culture of Canine Growth Plate Chondrocytes as a Model of Biomineralization
- Analysis of Chemical Interaction of 4-MET with Hydroxyapatite Using XPS
- Chemical Interaction between Titanium Implant Surface and Amino Acids
- New Surface Modification of Titanium Implant with Phospho-amino Acid
- Diagnosis, Prevention and Management for Aspiration Pneumonia in Dental Practice
- Synthetic Osteopontin-derived Peptide SVVYGLR can Induce Neovascularization in Artificial Bone Marrow Scaffold Biomaterials
- Advantages of TOF-SIMS Analysis of Hydroxyapatite and Fluorapatite in Comparison with XRD, HR-TEM and FT-IR
- 'Nectosome' : a novel cytoplasmic vesicle containing nectin in the egg of the sea urchin, Temnopleurus hardwickii
- FOMATION AND ACCUMULATION OF NECTIN-CONTAINING GRANULES DURING OOGENESIS IN THE SEA URCHIN, TEMNOPLEURUS HARDWICKI(Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- CHANGES IN THE CONCENTRATIONS OF PROSTAGLANDINS E AND F IN THE UTERO-PLACENTAL TISSUES OF LATE PREGNANT RABBIT AFTER THE ADMINISTRATION OF OXYTOCIN OR PROSTAGLANDIN F_
- LOCALIZATION AND KINETIC PROPERTIES OF PROSTAGLAND
- Hypothalamic Hamartoma Associated With Anterior Paraclinoid Aneurysm of the Internal Carotid Artery : Case Report
- Guideline for Denture Relining
- Effect of Noise on the Low Flow Rate Chaos in the Belousov-Zhabotinsky Reaction
- Cyclic ADP-Ribose Mediates Formyl Methionyl Leucyl Phenylalanine (fMLP)-Induced Intracellular Ca^ Rise and Migration of Human Neutrophils
- Controlled Administration of FGF-2 : A Prospective Drug Treatment for the Acceleration of Bone Regeneration
- Experimental Petit Mal-Like Seizure Induced by Microinjection of Kainic Acid Into the Unilateral Mediodorsal Nucleus of the Thalamus
- Disposable Dust Chamber for Home-visit Prosthodontic Treatments
- Intracranial Epidermoid Tumor After Subcutaneous Lipoma Excision
- TEM/EDX Observation of Micro-beads of Magnetically Activated Cell Sorting (MACS) System and Separation Trial of Mouse Mesenchymal Stem Cells from Bone Marrow Cells by MACS System
- Extremely High Drug-Reductase Activity Based on Aldehyde Oxidase in Monkey Liver
- Degree of immobilization of synthetic RGDS(PO_3H_2)PA peptides on titanium surfaces
- Participation of Hsp72 in DNA Damage by Dimethylarsenics : Possible Relationship between Hsp72 and Apoptosis Induced in Pulmonary Cultured Cells (Proceedings of the 23rd Symposium on Toxicology and Environmental Health)
- Involvement of Metabolic Methylation in Arsenic-Induced Mutagenesis for Mammalian Cells (Proceedings of the 22nd Symposium on Toxicology and Environmental Health)
- Gene Damage in Human Pulmonary Cultured Cells Induced by Dimethylarsenics (Proceedings of the 19th Symposium on Toxicology and Environmental Health)
- SEM Observation of Collagen Fibrils Secreted from the Body Surface of Osteoblasts on a CO_3apatite-collagen Sponge
- PURIFICATION AND CHARACTERIZATION OF CHYMOTRYPSIN-LIKE PROTEASES IN THE INTESTINE OF SEA URCHIN, H. PULCHERRIMUS(Biochemistry,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- First Derivative High-Tc SQUID Gradiometers with Narrow Structure from Single Layer YBCO Thin Film(Special Issue on Superconductive Electronics)
- Bone formation ability of carbonate apatite-collagen scaffolds with different carbonate contents
- Development of New Drug Delivery System for Implant Bone Augmentation Using a basic Fibroblast Growth Factor-gelatin Hydrogel Complex
- Development of Cell-hybrid Artificial Bone : Effect of Osteogenic Differentiation of Bone Marrow Stromal Stem Cells on Bone Formation with Newly Developed Interconnected Porous Calcium Hydroxyapatite
- Osteoblast Proliferation Behavior and Bone Formation on and in CO_3apatite-Collagen Sponges with a Porous Hydroxyapatite Frame
- Brain Metastasis in Malignant Pleural Mesothelioma Presenting as Intratumoral Hemorrhage : Case Report
- Detailed Consideration of Physicochemical Properties of CO_3apatites as Biomaterials in Relation to Carbonate Content Using ICP, X-ray Diffraction, FT-IR, SEM, and HR-TEM
- Oral motor function and masticatory performance in the community-dwelling elderly
- Acceleration of bone formation with BMP2 in frame-reinforced carbonate apatite-collagen sponge scaffolds
- Immobilization of Simulated Reducing Agent at the Surface of SiO_2 Fillers in Dental Composite Resins
- Cell Adhesion and Proliferation Patterns on Mixed Self-assembled Monolayers Carrying Various Ratios of Hydroxyl and Methyl Groups
- Higher Concentrations of Fluoride Ions Dramatically Inhibit the Survival of Osteoblasts
- Characterization of O-GlcNAcylation in Starfish (Asterina pectinifera) Development from Fertilization to Bipinnaria Larva
- Initial bone regeneration around fenestrated implants in Beagle dogs using basic fibroblast growth factor-gelatin hydrogel complex with varying biodegradation rates
- Inflammatory Myofibroblastic Tumor of the Cerebellar Hemisphere : Case Report
- Classification System for the Completely Dentate, Partial and Complete Edentulism
- Morphological and Biochemical Studies on Yolk Degradation in the Sea Urchin, Hemicentrotus pulcherrimus
- RELATIONS BETWEEN INTRANUCLEAR MITOTIC APPARATUS AND CONTRACTILE RING IN THE MARINE DINOFLAGELLATE, OXYRRHIS MARINA(Cell Biology and Morphology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- Elevated Serum DHEA-S Levels in Association with Hyperprolactinemia
- DECLINE IN SERUM RELAXIN LEVELS BEFORE LABOR IN WOMEN
- Relationship between Dental Occlusion and Falls among the Elderly with Dementia
- Subarachnoid Hemorrhage Caused by Pilocytic Astrocytoma : Case Report
- A High Density Sampling K-Ar Dating of the Kinpu-San Plutonic Body and the Initiation of the Philippine Sea Plate Subduction
- The relationship between tooth loss and cerebral stroke
- Resin Jig to Prevent Aspiration or Ingestion of Restorations
- Magnetoelectric Effects of Fe_3O_4 at 4.2K
- Radiant Heat Transfer in Adiabatic Tubular Reactor Containing Gray Gas
- The dynamic viscoelasticity and water absorption characteristics of soft acrylic resin materials containing adipates and a maleate plasticizer
- K-Ar dating studies of Ashigawa and Tokuwa granodiorite bodies and plutonic geochronology in the South Fossa Magna, central Japan
- A novel glass ionomer cement containing MgCO_3 apatite induced the increased proliferation and differentiation of human pulp cells in vitro
- Mutation Analysis of the KCNJ8 Gene in Japanese Patients with J-Wave Syndrome and Idiopathic Ventricular Fibrillation
- New development of carbonate apatite-chitosan scaffold based on lyophilization technique for bone tissue engineering
- Terminal-terminal types of liquid crystals. I. Synthesis and electrooptical properties of a ferroelectric liquid crystal.
- A Preliminary Study on a Newly Developed Cell-hybridized Artificial Bone in a Beagle Dog: Bone Marrow Stromal Cells with an Interconnected Porous Hydroxyapatite Scaffold
- Iron chelation therapy for a case of transfusion-independent MDS-RARS with significant iron overload
- Correlation between dental and nutritional status in community-dwelling elderly Japanese
- Comparative evaluation of bone regeneration using spherical and irregularly shaped granules of interconnected porous hydroxylapatite. A beagle dog study