Cyclic ADP-Ribose Mediates Formyl Methionyl Leucyl Phenylalanine (fMLP)-Induced Intracellular Ca2+ Rise and Migration of Human Neutrophils
スポンサーリンク
概要
- 論文の詳細を見る
Although cyclic ADP-ribose (cADPR), a novel Ca2+-mobilizing mediator, is suggested to be involved in the functions of neutrophils in rodents, its role in human neutrophils remains unclear. The present study examined the ability of cADPR to mobilize Ca2+ and mediate formyl methionyl leucyl phenylalanine (fMLP)-stimulated increase in cytosolic free Ca2+ concentration ([Ca2+]i) and migration in human neutrophils. cADPR induced Ca2+ release from digitonin-permeabilized neutrophils, and the release was blocked by 8Br-cADPR, an antagonist of cADPR. Immunophilin ligands, FK506 and rapamycin, but not cyclosporine A, inhibited cADPR-induced Ca2+ release. 8Br-cADPR partially reduced fMLP-induced [Ca2+]i rise and abolished the rise in combination with 2APB, an IP3-receptor antagonist. Anti-CD38Ab and NADase that interfere with cADPR formation, reduced the fMLP-induced [Ca2+]i rise. When β-NAD+, a substrate of ADP-ribosyl cyclase, and cADPR were added to the medium, the former gradually increased [Ca2+]i and the latter potentiated the fMLP-induced [Ca2+]i rise. The β-NAD+–induced [Ca2+]i rise in Ca2+-free medium was inhibited by anti-CD38Ab, 8Br-cADPR, FK506, ruthenium red, and thapsigargin. mRNAs of nucleoside transporter (NT), ENT1, ENT2, CNT, and CNT3 were expressed in neutrophils; and their inhibitors, inosine, uridine, and s-(4-nitrobenzyl)-6-thioinosine, reduced the [Ca2+]i rise induced by β-NAD+ and fMLP. fMLP-timulated migration was inhibited by the removal of Ca2+ from the medium or by the addition of 8Br-cADPR, anti-CD38Ab, NADase, and NT inhibitors. These results suggest that cADPR was synthesized extracellularly by CD38, transported into the cells through NTs, and then Ca2+ was mobilized by FK506-binding protein–dependent process. This process may be involved in fMLP-induced intracellular Ca2+ signaling and migration in human neutrophils.
著者
-
AKAGAWA Yasumasa
Department of Geriatric and Respiratory Medicine, Tohoku University Graduate School of Medicine
-
Morita Katsuya
Department of Dental Pharmacology, Hiroshima University Graduate School of Biomedical Sciences, Japa
-
Saida Minoru
Department of Advanced Prosthodontics, Hiroshima University Graduate School of Biomedical Sciences,
-
Morioka Norimitsu
Department of Dental Pharmacology, Hiroshima University Graduate School of Biomedical Sciences, Japa
-
Kitayama Tomoya
Department of Dental Pharmacology, Hiroshima University Graduate School of Biomedical Sciences, Japa
-
Dohi Toshihiro
Department of Dental Pharmacology, Hiroshima University Graduate School of Biomedical Sciences, Japa
-
Saida Minoru
Department Of Advanced Prosthodontics Hiroshima University Graduate School Of Biomedical Sciences
-
Morita Katsuya
Department Of Dental Pharmacology Hiroshima University Graduate School Of Biomedical Sciences
-
Morioka Norimitsu
Department Of Dental Pharmacology Hiroshima University Graduate School Of Biomedical Sciences
-
Kitayama Tomoya
Department Of Dental Pharmacology Hiroshima University Graduate School Of Biomedical Sciences
-
Akagawa Yasumasa
Department Of Advanced Prosthodontics Division Of Cervico-gnathostomatology Graduate School Of Biome
-
Dohi Toshihiro
Department of Dental Pharmacology, Division of Integrated Medical Science, Hiroshima University Graduate School of Biomedical Sciences, Japan
-
Morita Katsuya
Department of Dental Pharmacology, Hiroshima University Graduate School of Biomedical Sciences, Japan
-
Dohi Toshihiro
Department of Dental Pharmacology, Hiroshima University Graduate School of Biomedical Sciences, Japan
-
Kitayama Tomoya
Department of Dental Pharmacology, Hiroshima University Graduate School of Biomedical Sciences, Japan
-
Saida Minoru
Department of Advanced Prosthodontics, Hiroshima University Graduate School of Biomedical Sciences, Japan
関連論文
- Noradrenaline Induces Clock Gene Per1 mRNA Expression in C6 Glioma Cells Through β2-Adrenergic Receptor Coupled With Protein Kinase A – cAMP Response Element Binding Protein (PKA–CREB) and Src-Tyrosine Kinase – Glycogen Synthase Kinase-3β (Src–GSK-3β)
- Cyclic ADP-Ribose Mediates Formyl Methionyl Leucyl Phenylalanine (fMLP)-Induced Intracellular Ca2+ Rise and Migration of Human Neutrophils
- Dental status and mortality in institutionalized elderly people
- Dentin-derived Proteins Enhance Fibronectin-related but not Vitronectin-related Specific Adhesion of Human Periodontal Ligament Cells
- Mechanical Loading of Implants Prevents Resorption of Grafted Autogenous Bone in Maxilla : A Case Report of 3-year Clinical Follow-up
- Primary Culture of Canine Growth Plate Chondrocytes as a Model of Biomineralization
- Chemical Interaction between Titanium Implant Surface and Amino Acids
- New Surface Modification of Titanium Implant with Phospho-amino Acid
- Diagnosis, Prevention and Management for Aspiration Pneumonia in Dental Practice
- Delayed Infection of a Lymphocele Following Mastectomy with Immediate Breast Reconstruction : Report of a Case
- Involvement of Voltage-Gated Sodium Channel Na_v1.8 in the Regulation of the Release and Synthesis of Substance P in Adult Mouse Dorsal Root Ganglion Neurons
- Possible Involvement of Membrane Phospholipids in Inhibition by Ferrous Ions of [^3H]MK-801 Binding to the Native N-Methyl-D-Aspartate Channel in Rat Brain
- Guideline for Denture Relining
- Cyclic ADP-Ribose Mediates Formyl Methionyl Leucyl Phenylalanine (fMLP)-Induced Intracellular Ca^ Rise and Migration of Human Neutrophils
- Assessing an Eating Disorder Induced by 6-OHDA and the Possibility of Nerve Regeneration Therapy by Transplantation of Neural Progenitor Cells in Rats
- Controlled Administration of FGF-2 : A Prospective Drug Treatment for the Acceleration of Bone Regeneration
- Disposable Dust Chamber for Home-visit Prosthodontic Treatments
- Extremely High Drug-Reductase Activity Based on Aldehyde Oxidase in Monkey Liver
- Degree of immobilization of synthetic RGDS(PO_3H_2)PA peptides on titanium surfaces
- Cyclic ADP-Ribose Requires FK506-Binding Protein to Regulate Intracellular Ca^ Dynamics and Catecholamine Release in Acetylcholine-Stimulated Bovine Adrenal Chromaffin Cells
- Bone formation ability of carbonate apatite-collagen scaffolds with different carbonate contents
- Development of New Drug Delivery System for Implant Bone Augmentation Using a basic Fibroblast Growth Factor-gelatin Hydrogel Complex
- Development of Cell-hybrid Artificial Bone : Effect of Osteogenic Differentiation of Bone Marrow Stromal Stem Cells on Bone Formation with Newly Developed Interconnected Porous Calcium Hydroxyapatite
- Changes in Seizure Susceptibility to Local Anesthetics by Repeated Administration of Cocaine and Nomifensine but Not GBR12935: Possible Involvement of Noradrenergic System
- Oral motor function and masticatory performance in the community-dwelling elderly
- Liver abscess due to Lactococcus lactis cremoris
- Solitary Fibrous Tumor in the Pelvic Cavity with Hypoglycemia : Report of a Case
- Initial bone regeneration around fenestrated implants in Beagle dogs using basic fibroblast growth factor-gelatin hydrogel complex with varying biodegradation rates
- Cellular and Molecular Aspects of Monoamine Neurotransmitter Transporters
- Classification System for the Completely Dentate, Partial and Complete Edentulism
- Relationship between Dental Occlusion and Falls among the Elderly with Dementia
- STIMULATION OF CATECHOLAMINE RELEASE FROM ISOLATED ADRENAL GLANDS BY SOME AMINO ACIDS
- COMPARATIVE STUDIES OF HEPATIC NICOTINE METABOLIZING ENZYME ACTIVITIES IN MONKEYS AND DOGS
- Noradrenaline Induces Clock Gene Per1 mRNA Expression in C6 Glioma Cells Through β_2-Adrenergic Receptor Coupled With Protein Kinase A-cAMP Response Element Binding Protein (PKA-CREB) and Src-Tyrosine Kinase-Glycogen Synthase Kinase-3β (Src-GSK-3β)
- The relationship between tooth loss and cerebral stroke
- Resin Jig to Prevent Aspiration or Ingestion of Restorations
- The dynamic viscoelasticity and water absorption characteristics of soft acrylic resin materials containing adipates and a maleate plasticizer
- POTENT INHIBITORY ACTION OF PILOCARPINE ON HEPATIC DRUG METABOLISM
- New development of carbonate apatite-chitosan scaffold based on lyophilization technique for bone tissue engineering
- The anti-inflammatory effects of phenolic dental medicaments as determined by mouse ear edema assay.
- Volume Transmission of Substance P in Striatum Induced by Intraplantar Formalin Injection Attenuates Nociceptive Responses via Activation of the Neurokinin 1 Receptor
- Inhibition of Lipoxygenase by Phenolic compounds.
- Regulation by Protein Kinase C of Platelet-Activating Factor- and Thapsigargin-Induced Calcium Entry in Rabbit Neutrophils.
- Stimulation-evoked cyclic nucleotides efflux from isolated perfused dog adrenals and possible involvement of calcium.
- Changes in Convulsion Susceptibility of Lidocaine by Alteration of Brain Catecholaminergic Functions.
- Involvement of Brain Serotonergic Function in Lidocaine-Induced Convulsions in Mice.
- Pressure and temperature effects on octahedral-tetrahedral equilibria in pyridine solutions of some cobalt(II) halides.
- A Preliminary Study on a Newly Developed Cell-hybridized Artificial Bone in a Beagle Dog: Bone Marrow Stromal Cells with an Interconnected Porous Hydroxyapatite Scaffold
- Possible Involvement of Nitric Oxide in Acetylcholine-Induced Increase of Intracellular Ca2+ Concentration and Catecholamine Release in Bovine Adrenal Chromaffin Cells.
- Correlation between dental and nutritional status in community-dwelling elderly Japanese
- Volume Transmission of Substance P in Striatum Induced by Intraplantar Formalin Injection Attenuates Nociceptive Responses via Activation of the Neurokinin 1 Receptor
- Comparative evaluation of bone regeneration using spherical and irregularly shaped granules of interconnected porous hydroxylapatite. A beagle dog study