A Preliminary Study on a Newly Developed Cell-hybridized Artificial Bone in a Beagle Dog: Bone Marrow Stromal Cells with an Interconnected Porous Hydroxyapatite Scaffold
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概要
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<B>Purpose:</B> This study was performed to clarify the possibility of bone regeneration with a cell-hybridized artificial bone by evaluating bone formation in the beagle dog femur.<BR><B>Methods:</B> Bone marrow stromal cells (BMSCs) /interconnected porous hydroxyapatite (IP-CHA) composites as a cell-hybridized artificial bone were made by the injection of the BMSCs solution into IP-CHA. The distribution of BMSCs in IP-CHA was examined by scanning electron microscopy. Two BMSCs/IP-CHA composites (the BMSCs of one composite were labeled fluorescence) were placed into each of two bone sockets (<I>φ</I>3.7 mm×7 mm) prepared in the right femur of the beagle dog. After 4 weeks of surgery, new bone formation in the socket was examined by light microscopy and fluorescence microscopy.<BR><B>Results:</B> SEM showed well distribution of the injected BMSCs in the pores of IP-CHA. At 4 weeks, new bone formation was detected at the cortical areas of the composites, and injected BMSCs were identified in the new bone area of the interconnected pores. <BR><B>Conclusion:</B> The limited results of this study may suggest that BMSCs/IP-CHA composites as cell-hybridized artificial bone is a possible candidate for bone regeneration.
- 社団法人 日本補綴歯科学会の論文
著者
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KUBO Takayasu
Department of Advanced Prosthodontics, Division of Cervico-Gnathostomatology, Programs for Applied B
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DOI Kazuya
Department of Advanced Prosthodontics, Division of Cervico-Gnathostomatology, Programs for Applied B
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Akagawa Yasumasa
Department Of Advanced Prosthodontics Division Of Cervico-gnathostomatology Graduate School Of Biome
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HAYASHI Kazuhiko
Department of Advanced Prosthodontics, Division of Cervico-Gnathostomatology, Programs for Applied Biomedicine, Hiroshima University Graduate School of Biomedical Sciences
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DOI Kazuya
Department of Advanced Prosthodontics, Division of Cervico-Gnathostomatology, Programs for Applied Biomedicine, Hiroshima University Graduate School of Biomedical Sciences
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KUBO Takayasu
Department of Advanced Prosthodontics, Division of Cervico-Gnathostomatology, Programs for Applied Biomedicine, Hiroshima University Graduate School of Biomedical Sciences
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