Dentin-derived Proteins Enhance Fibronectin-related but not Vitronectin-related Specific Adhesion of Human Periodontal Ligament Cells

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In the early stage of periodontal ligament regeneration, specific adhesion of cells to the dentin surface is considered to be necessary. However, the mechanism of the event remains unclear. This study aimed to determine whether organic components in dentin may affect the specific adhesion of human periodontal ligament (hPDL) cells. Human PDL cells were isolated from extracted healthy human premolars. Guanidine-HCl extract of the dentin (DE) was prepared from freshly extracted bovine teeth. We analyzed the effect of the interaction between DE and the major cell adhesion molecules such as fibronectin (FN) and vitronectin (VN). The DE was sequentially over-coated onto FN- or VN-coated plates. Specific adhesion of hPDL cells was evaluated as the morphologic spread ratio. The results showed that the hPDL cell spread ratio significantly increased in the FN/DE-coated plates comparing to the FN-coated plates. On the other hand, no significant difference in spread ratio was detected between the VN/DE-coated and VN-coated plates. The results suggested that an unknown fibronectin specific cofactor exists in dentin matrix.
硬組織再生生物学会の論文