A Chimeric Tunicamycin Resistance Gene as a New Selectable Marker for Arabidopsis thaliana
スポンサーリンク
概要
- 論文の詳細を見る
Cultivation of genetically modified (GM) crops has been banned in many countries because of fears about their effects on human health and the environment. One reason cited by critics of these crops is the use of bacterial antibiotic resistance genes or herbicide resistance genes as selectable markers. To avoid using such genes, I employed the Arabidopsis thaliana gene encoding UDP-N-acetylglucosamine:dolichol phosphate N-acetylglucosamine-1-P transferase (GPT) as a selection marker in transformation of Arabidopsis. GPT catalyzes the initial reaction for the synthesis of asparagine-linked glycans that is inhibited by tunicamycin. Using the GPT gene in combination with tunicamycin functioned efficiently in the selection of transformed Arabidopsis. In addition, this selection was able to identify transformants at a very early stage post germination compared with the selection by kanamaycin. Application of this strategy to produce GM crops may help their acceptance by the public.
- 日本植物細胞分子生物学会の論文
- 2003-12-01
著者
-
KOIZUMI Nozomu
Research and Education Center for Genetic Information, Nara Institute of Science and Technology
-
Koizumi Nozomu
Research And Education Center For Genetic Information Nara Institute Of Science And Technology
-
Koizumi Nozomu
Research And Education Center For Genetic Information
関連論文
- Isolation and Characterization of a Putative Transducer of Endoplasmic Reticulum Stress in Oryza sativa
- IDENTIFICATION OF EARLY EXPRESSED GENES IN TOBACCO PLANTS DURING HYPERSENSITIVE RESPONSE UPON TMV INFECTION
- Cytosine methylation of chloroplast DNA in Chlamydomonas reinhardtii
- Screening of Wound-Responsive Genes Identifies an Immediate-Early Expressed Gene Encoding a Highly Charged Protein in Mechanically Wounded Tobacco Plants
- Differential Accumulation of Transcripts Encoding Sulfur Assimilation Enzymes upon Sulfur and/or Nitrogen Deprivation in Arabidopsis thaliana
- IDENTIFICATION AND CHARACTERIZATION OF SECRETED PROTEINS FROM CULTURED TOBACCO CELLS
- Cationic Peroxidases Secreted from Cultured Cells May Localize to Apoplasts in Tobacco Plant Roots
- Variable Interactions between Sucrose Non-fermented 1-Related Protein Kinases and Regulatory Proteins in Higher Plants(Biochemistry & Molecular Biology)
- An Arabidopsis SNF1-Related Protein Kinase, AtSR1, Interacts with a Calcium-Binding Protein, AtCBL2, of Which Transcripts Respond to Light
- Isolation and Responses to Stress of a Gene That Encodes a Luminal Binding Protein in Arabidopsis thaliana
- Isolation and analysis of cadmium responsive genes of Arabidopsis thaliana
- Purification and Characterization of Phosphoenolpyruvate Carboxylase of Photomixotrophically Cultured Green Tobacco Cells
- Sugar Responsible and Tissue Specific Expression of a Gene Encoding AtCIPK14, an Arabidopsis CBL-Interacting Protein Kinase
- A Chimeric Tunicamycin Resistance Gene as a New Selectable Marker for Arabidopsis thaliana