Optimal Conditions for Cultivation of Rhodococcus sp. N-774 and for Conversion of Acrylonitrile to Acrylamide by Resting Cells(Microbiorogy & Fermentation Industry)

概要

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The culture conditions for Rhodococcus sp. N-774 cells showing high nitrile hydratase activity and the reaction conditions for acrylamide production by the resting cells were optimized. Thiamine was essential for the growth of the strain. Yeast extract and Fe^<2+> or Fe^<3+> remarkably promoted the formation of nitrile hydratase of the cells. The reaction proceeded optimally at temperatures below 30℃. Incubation for 1 hr at above 40℃ resulted in inactivation of the enzyme. Through reaction at a temperature as low as 0℃, the inhibition and inactivation of the enzyme activity by the substrate, acrylonitrile, and the product, acrylamide, were remarkably reduced, and higher accumulation of acrylamide could be attained. Under the optimal conditions, a more than 20% (w/v) acrylamide solution was obtained with a conversion yield of nearly 100%. Thus, the aqueous acrylamide solution obtained showed a high enough quality for use for the commercial preparation of polyacrylamide.
社団法人日本農芸化学会の論文
1987-12-23