Detection of Biotin-Binding Immunoglobulin G in Human Sera Using Avidin-Coated Multiwell Microplates
スポンサーリンク
概要
- 論文の詳細を見る
Biotin-binding human immunoglobulin G(B-IgG)was detected by two different methods in the avidincoated multiwell format for the first time. In the first method, B-IgG was caught by avidin supported on the solid phase and was detected by alkaline phosphatase(ALP)-labeled anti-human IgG(method A). This method, however, was not able to distinguish B-IgG-positive and -negative human sera. The second method included peroxidase-labeled avidin instead of ALP-labeled anti-human IgG(method B). The sensitivity of method B was 28 times higher than that of method A. Method B in the multiwell-microplate format could detect B-IgG in the IgG fraction purified from human sera for the first time. This suggests that the level of B-IgG in human sera is very low.
- 公益社団法人日本薬学会の論文
- 2001-08-01
著者
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Kumasaka Kazunari
Department Of Laboratory Medicine Nihon University School Of Medicine
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Kumasaka Kazunari
Department Of Clinical Pathology Nihon University School Of Medicine
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Kumasaka Kazunari
Department Of Clinical Pathology Nihon University School Fo Medicine
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FUKUI Toru
Byotai-Seiri Laboratory
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Muratsugu Makoto
Department Of Medical Technology
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Muratsugu Makoto
Department Of Bioanalytical Science Osaka Prefecture College Of Health Sciences
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Tanaka Mami
Department of Bioanalytical Science, Osaka Prefecture College of Health Sciences
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Okushima Keiko
Department of Bioanalytical Science, Osaka Prefecture College of Health Sciences
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Tanaka Mami
Food Safety Research Institute Nissin Food Products Co. Ltd.
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Okushima Keiko
Department Of Bioanalytical Science Osaka Prefecture College Of Health Sciences
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Tanaka Mami
Department Of Bioanalytical Science Osaka Prefecture College Of Health Sciences
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FUKUI Toru
Byotai Seiri Laboratory
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