Biotin-Protein Ratios and Stability of Biotinylated Immunoglobulins as Standards for the Quantitation of Biotin-Binding Immunoglobulins(Analytical Biochemistry)
スポンサーリンク
概要
- 論文の詳細を見る
Biotin-binding IgG in human sera was quantitated using F(ab')_2anti-human IgG-coated multiwell microplates (Muratsugu, M. et al. 2003, Biol. Pharm. Bull., 26, 1605-1608). The biotin-protein ratio of biotinylated IgG, which was used as standard in the assay, was very important to quantitate the level of biotin-binding IgG. We investigated a synthesis method of biotinylated human immunoglobulins, how to determine the biotin-protein ratio of the biotinylated proteins, and their stability to prepare standards for measuring biotin-binding IgG, IgA, and IgM.
- 公益社団法人日本薬学会の論文
- 2006-07-01
著者
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FUKUI Toru
Byotai-Seiri Laboratory
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Honda Hidemi
Bioanalytical Science Laboratory Department Of Clinical Nutrition Osaka Prefecture University
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YAZAWA Ayaka
Bioanalytical Science Laboratory, Department of Clinical Nutrition, Osaka Prefecture University
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FUKUOKA Kyoko
Bioanalytical Science Laboratory, Department of Clinical Nutrition, Osaka Prefecture University
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MURATSUGU Makoto
Bioanalytical Science Laboratory, Department of Clinical Nutrition, Osaka Prefecture University
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Fukuoka Kyoko
Bioanalytical Science Laboratory Department Of Clinical Nutrition Osaka Prefecture University
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Yazawa Ayaka
Bioanalytical Science Laboratory Department Of Clinical Nutrition Osaka Prefecture University
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Muratsugu Makoto
Bioanalytical Sci. Lab. Dep. Of Clinical Nutrition Osaka Prefecture Univ.
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FUKUI Toru
Byotai Seiri Laboratory
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- Biotin-Protein Ratios and Stability of Biotinylated Immunoglobulins as Standards for the Quantitation of Biotin-Binding Immunoglobulins(Analytical Biochemistry)
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