Quantitation of Biotin-Binding Immunoglobulins G, A, and M in Human Sera Using F(ab')_2Anti-human Immunoglobulin-Coated Microplates(Analytical Biochemistry)
スポンサーリンク
概要
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Biotin-binding IgG (B-IgG) in human sera was quantified using previously developed F(ab')_2anti-human IgG-coated multiwell microplates (Muratsugu M. et al., 2003, Biol. Pharm. Bull., 26, 1605-1608). The levels of B-IgG in sera, however, were higher than those we predicted. In this study, we modified the assay using F(ab')_2anti-human IgG-coated multiwell microplates and successfully quantified the levels of B-IgG in sera. The cause of the unpredicted results was discussed in the text. In addition, the levels of biotin-binding IgA (B-IgA) and IgM (B-IgM) in sera could be measured using F(ab')_2anti-human IgA- or IgM-coated multiwell microplates. We quantified B-IgG, B-IgA, and B-IgM in sera from healthy specimens and patients with bronchial asthma, atopic dermatitis, epilepsy, and juvenile rheumatoid arthritis.
- 2008-03-01
著者
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FUKUI Toru
Byotai-Seiri Laboratory
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YAZAWA Ayaka
Bioanalytical Science Laboratory, Department of Clinical Nutrition, Osaka Prefecture University
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MURATSUGU Makoto
Bioanalytical Science Laboratory, Department of Clinical Nutrition, Osaka Prefecture University
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Yazawa Ayaka
Bioanalytical Science Laboratory Department Of Clinical Nutrition Osaka Prefecture University
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Muratsugu Makoto
Bioanalytical Sci. Lab. Dep. Of Clinical Nutrition Osaka Prefecture Univ.
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FUJIWARA Sami
Bioanalytical Science Laboratory, Department of Clinical Nutrition, Osaka Prefecture University
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NISHIDA Satsuki
Bioanalytical Science Laboratory, Department of Clinical Nutrition, Osaka Prefecture University
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Fujiwara Sami
Bioanalytical Science Laboratory Department Of Clinical Nutrition Osaka Prefecture University
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Nishida Satsuki
Bioanalytical Science Laboratory Department Of Clinical Nutrition Osaka Prefecture University
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FUKUI Toru
Byotai Seiri Laboratory
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