Comparison of the Solid Phase-supported Receptor and Ligand Competitive Assays for Biotin

スポンサーリンク

概要

• 論文の詳細を見る

• Solid phase-supported receptor and ligand competitive assays for biotin were tested with respect to a mathematical model. We could assume the same mathematical model for both the solid phase-supported receptor and ligand competitive assays. In addition, we could eliminate the so-called blocking procedure in these assays. The amount of immobilizing reagent (biotinylated bovine serum albumin, BBSA) in the solid phase-supported ligand competitive assay was less than that (avidin) in the solid phase-supported receptor competitive assay. Desthiobiotin and biocytin influenced the solid phase-supported ligand competitive assay, but lipoic acid and lysine did not.

• 公益社団法人　日本薬学会の論文

著者

• Muratsugu Makoto

  Bioanalytical Sci. Lab. Dep. Of Clinical Nutrition Osaka Prefecture Univ.

• Amano Namiko

  Bioanalytical Science Laboratory, Department of Clinical Nutrition, Osaka Prefecture University

• Takeda Rie

  Bioanalytical Science Laboratory, Department of Clinical Nutrition, Osaka Prefecture University

関連論文

• Biotin-Protein Ratios and Stability of Biotinylated Immunoglobulins as Standards for the Quantitation of Biotin-Binding Immunoglobulins(Analytical Biochemistry)
• The Total Biotin Level in Dried Soybeans Treated with Hydrochloric Acid Quantified Using an Agar Plate Bioassay
• Quantitation of Biotin-Binding Immunoglobulins G, A, and M in Human Sera Using F(ab')_2Anti-human Immunoglobulin-Coated Microplates(Analytical Biochemistry)
• Quantitative Measurement of Biotin-Binding Immunoglobulin G in Human Sera Using F(ab')_2anti-human IgG-Coated Multi-well Plates(Analytical Biochemistry)
• Detection of Antibody and the Antigen Classes in Circulating Immune Complexes Fractionated with the Sucrose Density Gradient Centrifugation Using an F(ab')_2anti-C3 ELISA
• Comparison of the Solid Phase-supported Receptor and Ligand Competitive Assays for Biotin

スポンサーリンク