Quantitative Measurement of Biotin-Binding Immunoglobulin G in Human Sera Using F(ab')_2anti-human IgG-Coated Multi-well Plates(Analytical Biochemistry)
スポンサーリンク
概要
- 論文の詳細を見る
Biotin-binding human immunoglobulin G (B-IgG) was quantitatively measured using an F(ab')2anti-human IgG-coated multi-well microplate for the first time. B-IgG was caught by F(ab')_2anti-human IgG and was detected by the following detecting reagents: Peroxidase-labeled streptavidin, avidin and peroxidase-biotin, or avidin-biotinylated peroxidase complex with method A, B, or C, respectively. Commercially available B-IgG was detected by all these three methods. However, method A and B could not detect B-IgG in the human sera used in this study, but we quantitatively measured the B-IgG level using method C. The result is probably due to the fact that the sensitivity of method C was higher than that of methods A or B. Properties of B-IgG detected by method C are discussed in the text.
- 公益社団法人日本薬学会の論文
- 2003-11-01
著者
-
Fukui T
Hoshi Univ. Tokyo Jpn
-
Fukui T
Byotai‐seiri Lab. Tokyo Jpn
-
FUKUI Toru
Byotai-Seiri Laboratory
-
Fukui T
Byotai-seiri Laboratory
-
MURATSUGU Makoto
Bioanalytical Science Laboratory, Department of Clinical Nutrition, Osaka Prefecture University
-
Fukui T
Department Of Materials Science And Engineering Faculty Of Engineering Kyushu University
-
Muratsugu M
Bioanalytical Science Laboratory Department Of Clinical Nutrition Osaka Prefecture College Of Nursin
-
MURAMOTO Eri
Bioanalytical Science Laboratory, Department of Clinical Nutrition, Osaka Prefecture College of Nurs
-
Muratsugu Makoto
Bioanalytical Sci. Lab. Dep. Of Clinical Nutrition Osaka Prefecture Univ.
-
Muramoto Eri
Bioanalytical Science Laboratory Department Of Clinical Nutrition Osaka Prefecture College Of Nursin
-
FUKUI Toru
Byotai Seiri Laboratory
関連論文
- Effects of Development on Acetoacetyl-CoA Synthetase Biosynthesis in Rat Liver
- Biological Activity of β-Dolabrin, γ-Thujaplicin, and 4-Acetyltropolone, Hinokitiol-Related Compounds(Biochemistry/Molecular Biology)
- Biological Activity of α-Thujaplicin, the Isomer of Hinokitiol(Biochemistry/Molecular Biology)
- Effects on M5076-Hepatic Metastasis of Retinoic Acid and N-(4-Hydroxyphenyl) Retinamide, Fenretinide Entrapped in SG-Liposomes
- L-Tryptophan as an Antioxidant in Human Placenta Extract
- Antioxidant Properties of a New Type of Polyene, Falconensone A and Its Derivatives
- Induction of Apoptosis in the Human Promyelocytic Leukemia Cell Line HL60 by Falconensone A and Its Derivatives, New Polyenes
- Suppressive Effects of Uracil, Tyrosine, and Phenylalanine Contained in Human-Placenta Extract on Acute Ethanol-Induced Liver Injury in Mice
- Purification and Identification of Antioxidant Substances in Human-Placenta Extracts
- Studies on the Suppressive Effect of Antioxidant in Human Placental Extract on Oxidative Stress and its Mechanism(PROCEEDINGS OF 24TH SYMPOSIUM ON TOXICOLOGY AND ENVIRONMENTAL HEALTH)
- The Energetic Particle Spectrometer HEP onboard the GEOTAIL Spacecraft
- Formation of Retinoyl-CoA in Rat Tissues
- Novel Biological Activity of the Region (106-126) on Human Prion Sequence
- Biotin-Protein Ratios and Stability of Biotinylated Immunoglobulins as Standards for the Quantitation of Biotin-Binding Immunoglobulins(Analytical Biochemistry)
- Detection of Biotin-Binding Immunoglobulin G in Human Sera Using Avidin-Coated Multiwell Microplates
- HVEM study of crack-tip dislocations in Si crystals prepared by FIB and twin-blade cutting method
- TEM/AFM Observation of Crack Tip Plasticity in Silicon Single Crystals
- The Total Biotin Level in Dried Soybeans Treated with Hydrochloric Acid Quantified Using an Agar Plate Bioassay
- Quantitation of Biotin-Binding Immunoglobulins G, A, and M in Human Sera Using F(ab')_2Anti-human Immunoglobulin-Coated Microplates(Analytical Biochemistry)
- Adaptation to Stress when Considered in Relation to Food Intake
- Quantitative Measurement of Biotin-Binding Immunoglobulin G in Human Sera Using F(ab')_2anti-human IgG-Coated Multi-well Plates(Analytical Biochemistry)
- Contribution of Nitric Oxide to Potassium Bromate-Induced Elevation of Methaemoglobin Concentration in Mouse Blood(Toxicology)
- Detection of Antibody and the Antigen Classes in Circulating Immune Complexes Fractionated with the Sucrose Density Gradient Centrifugation Using an F(ab')_2anti-C3 ELISA
- Comparison of the Solid Phase-supported Receptor and Ligand Competitive Assays for Biotin
- Agar Plate Method Using Lactobacillus plantarum for Biotin Determination in Serum and Urine.