Construction of a Promoter Probe Vector Autonomously Maintained in Aspergillus and Characterization of Promoter Regions Derived from A. niger and A. oryzae Genomes
スポンサーリンク
概要
- 論文の詳細を見る
We used a plasmid carrying a sequence for autonomous maintenance in Aspergillus (AMAl) and the E. coli uidA gene as a reporter gene to search the A. oryzae and A. niger genomes for DNA fragments having strong promoter activity. β-glucuronidase (GUS)-producing A. oryzae transformants containing the No. 8AN derived from A. nigei, or the No. 9AO derived from A. oryzae, were constitutive for the expression of the uidA gene when cultivated in the prcdence of a variety of carbon and nitrogen sources. When the GUS-producing transformants were grown in liquid culture, the No. 8AN showed an increase of approximately 3-fold in GUS activity compared to the amyB (α-amylase encoding gene) promoter. There was also a correcponding increase in the amount of GUS gene-specific mRNA. When these transformants were grown as rice-koji, the No. 8AN showed an increase of approximately 6-fold compared to the amyB promoter, and the amount of GUS protein produced also increased. These strong promoter regions might be applicable to the production of other heterologous proteins in Aspergillus species.
- 社団法人日本農芸化学会の論文
- 1996-03-23
著者
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Ozeki K
Ozeki Corp. Hyogo Jpn
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Ozeki Kenji
General Research Laboratory Ozeki Corp.
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Ozeki Kenji
General Research Laboratories Ozeki Corp.
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HAMACHI Masaaki
General Research Laboratory, Ozeki Corp
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Kanda Akihiro
Process And Systems Development Group Production Technology Center Kaneka Corporation
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KANDA Akihiro
General Research Laboratory, Ozeki Corporation
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NUNOKAWA Yataro
General Research Laboratory, Ozeki Corporation
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Kanda Akihiro
General Research Laboratory Ozeki Corporation
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Nunokawa Y
General Research Laboratory Ozeki Corporation
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Ohbuchi K
Ozeki Corp. Hyogo Jpn
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Hamachi M
Ozeki Corp. Hyogo Jpn
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Hamachi Masaaki
General Research Laboratory Ozeki Corp.
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