Purification and Characterization of an Extracellular Metalloprotease from Pseudomonas fluorescens.
スポンサーリンク
概要
- 論文の詳細を見る
An extracellular metalloprotease was purified from the culture supernatant of Pseudomonas fluorescens strain KT1 to apparent homogeneity and shown to consist of a single polypeptide chain (Mr, 46, 000-47, 000). The enzyme was strongly inhibited by chelating agents such as EDTA and o-phenanthroline, and activated by certain detergents. Among the peptidyl 4-methylcoumaryl-7-amide (MCA) substrates examined, t-butyloxycarbonyl-Arg-Val-Arg-Arg-MCA was the best one. With this substrate, the enzyme exhibited a pH optimum of around pH 5.5 in the absence of Co2+ ions, whereas it showed two different pH optima (at pHs around 5.5 and 8-9) in the presence of Co2+ ions due to remarkable activation by Co2+ ions in the alkaline pH range. On the other hand, a single broad pH optimum of around 6 to 8 was obtained with some peptides in both the presence and absence of Co2+ ions, and no activation by Co2+ was observed. The enzyme showed trypsin-like specificity, preferentially cleaving certain arginyl peptide bonds, and hydrolyzed the basic protein, histone, most rapidly among various proteins examined. Partial amino acid sequence analysis revealed that the enzyme is highly homologous with proteases of the serralysin family, a group of zinc metalloproteases.
- 社団法人 日本生化学会の論文
著者
-
Jeohn Gwang-ho
Department Of Biophysics And Biochemistry Graduate School Of Science The University Of Tokyo
-
KIM Hee-Jung
Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo
-
Tamanoue Yoshiaki
Department Of Biophysics And Biochemistry Graduate School Of Science The University Of Tokyo
-
Yokota Akira
Institute For Fermentation
-
Takahashi Takayuki
Department Of Biophysics And Biochemistry Graduate School Of Science University Of Tokyo
-
Takahashi Kenji
Department Of Applied Biological Chemistry Graduate School Of Agriculture Kinki University
-
IWAMATSU Akihiro
Central Lab. Key Tech. , Kirin Brewery Co.
-
Kim Yong-Tae
Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo
関連論文
- Successful Allogeneic Bone Marrow Transplantation for Diamond-Blackfan Anemia Complicated by Severe Cardiac Dysfunction due to Transfusion-Induced Hemochromatosis
- 排卵機序に関わるプロテアーゼ
- Multicenter Prospective Study of Clonal Complications in Adult Aplastic Anemia Patients following Recombinant Human Granulocyte Colony-Stimulating Factor (Lenograstim) Administration
- Chemotaxonomy of planktonic cyanobacteria based on non-polar and 3-hydroxy fatty acid composition
- Chronic Eosinophilic Leukemia with the FIP1L1-PDGFRα Fusion Gene in a Patient with a History of Combination Chemotherapy
- Toxicity and Bioaccumulation of Hexavalent Chromium in Green Paramecium, Paramecium bursaria
- メダカエンテロペプチダーゼの発見 : バイオツールとしての利用
- 排卵酵素 : 排卵研究の歴史とメダカ排卵酵素の同定
- Physiological and Chemotaxonomical Studies on Microflora within a Composter Operated at High Temperature
- Histiocytic sarcoma and underlying chronic myelomonocytic leukemia : a proposal for the developmental classification of histiocytic sarcoma
- Successful allogeneic bone marrow transplantation for myelodysplastic syndrome complicated by severe pulmonary alveolar proteinosis
- Refractory de novo myeloid sarcoma : a case report and therapeutic strategy based on bone marrow minimal residual disease
- Multiple Granulocytic Sarcomas in Essential Thrombocythemia
- Multiple Extranodal Tumors in μ-Heavy Chain Disease
- ヒトフォリプシンの精製およびその解析
- Expression of cyclooxygenase-2 and prostaglandin receptor EP4b mRNA in the ovary of the medaka fish, Oryzias latipes: Possible involvement in ovulation
- Mucinous Cystadenocarcinoma in the Appendix in a Patient with Nonrotation : Report of a Case
- The Authors Reply: POEMS Syndrome, Congestive Heart Failure and Thalidomide -Food for Thought
- Successful Treatment of POEMS Syndrome Complicated by Severe Congestive Heart Failure with Thalidomide
- ブタ卵巣卵胞液中に存在する一本鎖前駆体フォリプシンの同定
- Formation of Root Nudules by Rhizobium huakuii biovar. renge bv. nov. on Astragalus sinicus cv. Japan
- 生殖器官に特異的な新規MMPのcDNAクローニング
- Production of Sedoheptulose by Bacillus subtilis
- Group I Intron Located in PR Protein Homologue Gene in Youngia japonica
- Evolutionary relationships of members of the genera Taphrina, Protomyces, Schizosaccharomyces, and related taxa within the archiascomycetes : Integrated analysis of genotypic and phenotypic characters
- 生殖医学に役立つメダカの排卵研究
- メダカの in vitro 排卵実験系を利用した排卵研究
- Expression and Localization of Prolyl Oligopeptidase in Mouse Testis and Its Possible Involvement in Sperm Motility(Reproductive Biology)
- EXPRESSION AND LOCALIZATION OF PROLYL OLIGOPEPTIDASE IN MOUSE TESTIS AND BRAIN(Biochemistry)Proceedings of the Seventy-First Annual Meeting of the Zoological Society of Japan
- Purification and Further Characterization of Enteropeptidase from Porcine Duodenum
- 哺乳類の排卵機構に関する研究
- 435 ヒト卵胞液中に存在する血漿カリクレイン様セリン・プロテアーゼ「フォリプシン」の活性化および排卵への関与について
- Purification and Characterization of an Extracellular Metalloprotease from Pseudomonas fluorescens^1
- Molecular Mechanism of Follicle Rupture during Ovulation
- Occurrence of a Latent Serine Protease in the Follicular Fluid of Porcine Ovary
- 卵巣の濾胞成熟とプロテア-ゼ
- Occurrence of a Novel 350-kDa Serine Proteinase in the Fluid of Porcine Ovarian Follicles and Its Increase during Their Maturation(Reproductive Biology)
- 哺乳類のプロリン特異的ペプチダ-ゼ--消化管での蛋白質分解以外に生殖器官での生理機能に興味(今日の話題)
- カテプシンB--最近の研究と将来の問題
- Trehalose Production by a Strain of Micrococcus varians
- Gordonan, an Acidic Polysaccharide with Cell Aggregation-Inducing Activity in Insect BM-N4 Cells, Produced by Gordonia sp.
- Primary Myelofibrosis Terminated in Basophilic Leukemia and Successful Allogeneic Bone Marrow Transplantation
- Successful Treatment of μ-Heavy Chain Disease with Fludarabine Monophosphate : A Case Report
- Association of Intestinal Malignant Lymphoma and Ulcerative Colitis
- Involvement of the NUP98 Gene in a Chromosomal Translocation t(11;20)(p15;q11.2) in a Patient With Acute Monocytic Leukemia (FAB-M5b)
- Expression of Bradykinin B_2 Receptor in the Mouse Ovary(Physiology)
- Characterization of mouse tissue kallikrein 5
- ANALYSIS OF MEDAKA KALLIKREIN LIKE PROTEIN THE RE PRODUCTIVE ORGANS
- MECHANISM OF OVULATION IN VERTEBRATES : SEARCHING FOR PROTEOLYIC ENZYMES RESPONSIBLE FOR DEGRADATION OF FOLLICLE WALLS IN FOLLICLE RUPTURE USING MEDAKAFISH
- Expression of matrix metalloproteinases in the ovary of the medaka fish oryzias laipes
- EFFECT OF BRADYKININ ON MMP EXPRESSION IN MOUSE OVARY(Endocrinology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- IDENTIFICATION OF THE FOLLICLES DIGESTION FACTOR USING THE MEDAKA FISH IN VITRO OVULATION SYSTEM(Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Cloning and Characterization of Stromelysin-3 expressed in the Ovary of the Medaka fish OLYZIAS LATIPES(Biochemistry)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- CLONING AND CHARACTERIZATION OF COLLAGENASE-LIKE MMP EXPRESSED IN MEDAKA FISH OVARY(Biochemistry)Proceedings of the Seventy-First Annual Meeting of the Zoological Society of Japan
- EXPRESSION AND LOCALIZATION OF BRADYKININ B2 RECEPTOR IN THE MOUSE OVARY(Endocrinology)Proceedings of the Seventy-First Annual Meeting of the Zoological Society of Japan
- CHARACTERIZATION OF THE BRADYKININ TYPE 2 RECEPTOR IN PORCINE OVARY(Endocrinology)Proceedings of the Seventy-First Annual Meeting of the Zoological Society of Japan
- MATRIX METALLOPROTEINASES IN MEDAKA FISH OVARY AND THEIR EXPRESSION UPON OVULATION(Biochemistry)(Proceedings of the Seventieth Annual Meeting of the Zoological Society of Japan)
- Release of a Newly-Identified Cysteine protease, Tetrain, from Tetrahymena into Culture Medium during the Cell Growth^1
- Symbiotic Algae-free Strains of The Green Paramecium Paramecium bursaria Produced by Herbicide Paraquat
- Formation of 1-Deoxy-ketoses by Pyruvate Dehydrogenase and Acetoin Dehydrogenase(Microbiology & Fermentation Industry)
- Castleman's disease of the abdomen and pelvis: Report of three cases and a review of the literature
- ENZYMATIC AND BIOCHEMICAL CHARACTERIZATION OF RECOMBINANT MOUSE GLANDULAR KALLIKREIN 6(Biochemistry,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- IDENTIFICATION OF KEY ENZYMES RESPONSIBLE FOR FOLLICLE RUPTURE AND ELUCIDATION OF MECHANISM OF FOLLICLE RUPTURE DURING OVULATION IN MEDAKA(Biochemistry,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- Biochemical characterization of human kallikrein 8 and its possible involvement in the degradation of extracellular matrix proteins
- Single-chain tissue-type plasminogen activator is a substrate of mouse glandular kallikrein 24
- Gelatinase A and membrane-type matrix metalloproteinases 1 and 2 are responsible for follicle rupture during ovulation in the medaka
- CLONING OF A NOVEL SERINE PROTEASE FROM MEDAKA OVARY(Biochemistry,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- ANALYSIS OF PLASMINOGEN ACTIVATOR/PLASMIN SYSTEM IN REPRODUCTIVE ORGANS OF ORYZIAS LATIPES(Biochemistry,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- EVIDENCE FOR INVOLVEMENT OF MT2-MMP IN THE DEGRADATION OF FOLLICLE WALL DURING OVULATION IN MEDAKA FISH(Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- Nocardioides exalbidus sp. nov., a novel actinomycete isolated from lichen in Izu-Oshima Island, Japan
- Electromyographical Evaluation of Muscle Activity by Occlusal Tooth Contact
- Hepatosplenic αβ T cell lymphoma
- Production of Idoheptulosan from Sedoheptulosan by Microorganisms
- PB-28 A New Bioassy For Acrylamide Toxicity Using Protozoa, Paramecium bursaria(Monitoring,Poster presentation B)
- EFFECTS OF ACRYLAMIDE ON A SYMBIOTIC ASSOCIATION OF SYMBIOTIC ALGAE WITH THE CILIATE PARAMECIUM BURSARIA(Cell Biology and Morphology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- Presence of a Tetrahymena growth promoting activity in fetal bovine serum
- Purification and Gene Cloning of a Chitosanase from Bacillus ehimensis EAG1
- Use of Paramecium Species in Bioassays for Environmental Risk Management: Determination of IC_ Values for Water Pollutants
- Identification and Characterization of Lactococcal and Acetobacter Strains Isolated from Traditional Caucasusian Fermented Milk
- The Serum Cytokine Profiles of Lymphoma-Associated Hemophagocytic Syndrome : A Comparative Analysis of B-Cell and T-Cell/Natural Killer Cell Lymphomas
- Management of two pregnancies in a woman with mixed connective tissue disease, pulmonary fibrosis, frequent pneumothorax and oxygen inhalation therapy along with a published work review
- ANALYSIS OF MEDAKA KALLIKREIN : COMPARISON WITH MOUSE KALLIKREINS
- STUDIES ON THE INVOLVEMENT OF THE tPA/PLASMIN SYSTEM, CATHEPSIN L, AND ADAMTS-1 IN FOLLICLE RUPTURE DURING OVULATION IN MEDAKA
- Purification and Characterization of a Major Collagenase from Streptomyces parvulus
- Rhodococcus baikonurensis BTM4c, a Boron-Tolerant Actinobacterial Strain Isolated from Soil
- Control of a Pendulum Under Torque Limitation : 2nd Report, Energy Equation Approach to Swinging-Up Motion and Experiments
- Epidemiological study of ulcerative colitis in Japan: Incidence and familial occurrence
- メダカ卵巣におけるシクロオキシゲナーゼ-2(COX-2)及びプロスタグランジンE_2受容体EP4bの発現と排卵への関与
- Isolation and Characterization of Bacteriophage Induced from a New Isolate of Thermus aquaticus
- New Subspecies of Genus Streptosporangium, Streptosporangium amethystogenes subsp. fukuiense subsp. nov.
- CHARACTERIZATION OF A BRADYKININ RECEPTOR IN THE FOLLICLES OF MOUSE OVARY AND ITS CHANGES IN THE EXPRESSION DURING ESTROUS CYCLE(Endocrinology)(Proceedings of the Seventieth Annual Meeting of the Zoological Society of Japan)
- Expression and localization of collagen type IV α1 chain in medaka ovary
- New evidence for the involvement of prostaglandin receptor EP4b in ovulation of the medaka, Oryzias latipes.
- Systemic Follicular Lymphoma with Massive Intestinal Involvement with Leukemic Manifestation
- Enteropathy-Associated T-Cell Lymphoma Type II Complicated by Autoimmune Hemolytic Anemia
- Electronic and Excitonic Structures of Inorganic–Organic Perovskite-Type Quantum-Well Crystal (C4H9NH3)2PbBr4
- Formation of Abdominal Cerebrospinal Fluid Pseudocyst : Case Report
- Leukemic Manifestation of Blastic Plasmacytoid Dendritic Cell Neoplasm Lacking Skin Lesion : A Borderline Case between Acute Monocytic Leukemia
- Polymyositis as a Paraneoplastic Syndrome in Cytotoxic Molecule-positive and Epstein-Barr Virus-associated Peripheral T-cell Lymphoma, Not Otherwise Specified
- Purification and Characterization of an Extracellular Metalloprotease from Pseudomonas fluorescens.