Purification and Gene Cloning of a Chitosanase from Bacillus ehimensis EAG1
スポンサーリンク
概要
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Bacillus ehimensis EAG1(IFO15659)produced and secreted chitosanase in the presence of exogenous chitosan. The chitosanase was purified from the culture filtrate of the bacterium to apparent homogeneity in SDS-polyacrylamide gel electrophoresis. The purified enzyme had a molecular weight of approximately 31, 000. A1.9-kbp DNA fragment containing the chitosanase gene was cloned and the complete nucleotide sequence was determined. The sequence was found to contain a single open reading frame encoding a protein of 302 amino acids. The deduced amino acid sequence showed significant homology with the chitosanase from Bacillus circulans MH-K1.
- 社団法人日本生物工学会の論文
- 1999-03-25
著者
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YOKOTA Akira
Institute of Molecular and Cellular Biology, University of Tokyo
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SAKANE Takeshi
Pharmaceutical Discovery Center, Pharmaceutical Research Division, Takeda Chemical Industries, Ltd.
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Yokota Akira
Institute Of Molecular And Cellular Biosciences The University Of Tokyo
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Fujita Tadashi
Faculty Of Agriculture Ehime University
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Sakane Takeshi
Pharmaceutical Discovery Center Pharmaceutical Research Division Takeda Chemical Industries Ltd.
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Sakane Takeshi
Pharmaceuticals Research Division Takeda Chemical Industries Ltd.
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AKIYAMA KOUICHI
Faculty of Agriculture, Ehime University
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KUROSHIMA KEN-ICHI
Pharmaceuticals Research Division, Takeda Chemical Industries Ltd.
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TAKATA RENKICHI
Faculty of Agriculture, Ehime University
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Yokota Akira
Institute For Fermentation
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Akiyama Kouichi
Faculty Of Agriculture Ehime University
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Takata Renkichi
Faculty Of Agriculture Ehime University
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Kuroshima Ken-ichi
Pharmaceuticals Research Division Takeda Chemical Industries Ltd.
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