Synthesis of Rubisco gene products is upregulated by increasing the copy number of rbcL gene in Chlamydomonas chloroplast genome, without increased accumulation of the two Rubisco subunits
スポンサーリンク
概要
- 論文の詳細を見る
In an attempt to increase the amount of Rubisco in a green alga, the Chlamydomonas reinhardtii rbcL gene connected to the aadA cassette was integrated into chloroplast inverted repeats in this organism to generate a transformant with three copies of rbcL (3L). Synthesis and accumulation of the rbcL transcript in 3L were 2.3 and 1.4 times those of the control, respectively. Although synthesis of the large subunit (LSU) in 3L was 2.6 times that in the control, accumulation of the LSU was only 1.1 times that in the control. Synthesis of rbcS transcript and the small subunit (SSU) in 3L was also upregulated to 1.7 and 1.3 times those in the control, respectively, while their accumulation was unaffected. These results suggest that an increase in rbcL gene number upregulates transcription of rbcL from chloroplast genomes and that of rbcS from a nuclear genome, but does not affect the accumulation of LSU and SSU.
- 日本植物細胞分子生物学会の論文
- 2005-06-01
著者
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UCHIDA Hidenobu
School of Environmental Science and Engineering, Kochi University of Technology
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YAMASHITA Hirofumi
Graduate School of Human and Environmental Sciences, Kyoto Prefectural University
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Ohyama Kiyoshi
Plant Science Center Riken
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IWANO Megumi
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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FUKUZAWA Hideya
Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University
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Ohyama Kanji
Department Of Chemistry And Materials Science Tokyo Institute Of Technology
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ISONO Kyoichi
Laboratory of Plant Molecular Physiology, RITE
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OHYAMA Kanji
Institute of Bioresources and Biotechnology, Ishikawa Prefectural University
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UCHIDA Hidenobu
Laboratory of Plant Molecular Physiology, RITE
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TOMIZAWA Ken-Ich
Laboratory of Plant Molecular Physiology, RITE
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YAMASHITA Hirofumi
Laboratory of Applied Biology, Faculty of Human Environment, Kyoto Prefectural University
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YOKOTA Akiho
Laboratory of Plant Molecular Physiology, RITE
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Ohyama Kanji
Res. Inst. For Bioresources And Biotechnology Ishikawa Prefectural Univ.
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Ohyama K
Kyoto Univ. Kyoto Jpn
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Ohyama Kanji
Div. Appl. Life Sci. Grad. Schl. Agr. Kyoto Univ.
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Ohyama Kanji
Div. Of Appl. Life Sci. Grad. Sch. Of Agric. Kyoto Univ.
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Ohyama Kanji
Graduate School Of Biological Sciences Nara Institute Of Science And Technology:laboratory Of Plant
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Iwano Megumi
Nara Inst. Of Sci. And Technol. Nara Jpn
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Iwano Megumi
Graduate School Of Biological Science Nara Advanced Institute Of Science And Technology
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Fukuzawa Hideya
Div. Of Integrated Life Sci. Graduate School Of Biostudies Kyoto Univ.
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Fukuzawa H
Lab. Of Plant Molecular Biology Graduate School Of Biostudies Kyoto Univ.
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Fukuzawa Hideya
Div. Of Appl. Life Sci. Grad. Sch. Of Agric. Kyoto Univ.
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Fukuzawa Hideya
Division Of Integrated Life Science Graduate School Of Biostudies Kyoto University
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Uchida Hidenobu
School Of Environmental Sci. And Engineering Kochi Univ. Of Technol.
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Uchida Hidenobu
School Of Environmental Science And Engineering Kochi University Of Technology
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Yamashita Hirofumi
Graduate School Of Human And Environmental Sciences Kyoto Prefectural University
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Tomizawa Ken-ichi
Laboratory Of Plant Molecular Physiology Rite
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Ohyama Kanji
Laboratory Of Plant Molecular Biology Department Of Agricultural Chemistry Faculty Of Agriculture Ky
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Yokota Akiho
Laboratory Of Plant Molecular Physiology Rite
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Ohyama Kanji
Institute Of Bioresources And Biotechnology Ishikawa Prefectural University
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Ohyama Kanji
Dept.agric.chem. Fac.agric. Kyoto Univ.
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Isono Kyoichi
Laboratory Of Plant Molecular Physiology Rite
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Isono Kyoichi
Laboratory Of Plant Cell Technology Graduate School Of Nutritional And Environmental Sciences Univer
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