Agrobacterium-Mediated Transformation of Euphorbia tirucalli Callus

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概要

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• In order to establish a basis for transformation technology in the petroleum plant Euphorbia tirucalli, the callus of the plant was infected with Agrobacterium, washed with distilled water, sterilized with distilled water containing 100 mg/l of carbenicillin, selected on solidified B5 medium containing 13 mg/l of G418 and 100 mg/l of carbenicillin, and then on solidified B5 medium containing 25 mg/l of G418 and 100 mg/l of carbenicillin for the transgenic calli, and then the callus lines were subcultured successively on solidified B5 medium containing 50 mg/l of G418. We performed PCR analysis of sterilized G418-resistant callus line DNA and concluded that the gene introduced was integrated into the callus genome.

• 社団法人 日本農芸化学会の論文

著者

• UCHIDA Hidenobu

  School of Environmental Science and Engineering, Kochi University of Technology

• YAMASHITA Hirofumi

  Graduate School of Human and Environmental Sciences, Kyoto Prefectural University

• MURANAKA Toshiya

  RIKEN Plant Science Center

• OHYAMA Kanji

  Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University

• Uchida Hidenobu

  School Of Environmental Sci. And Engineering Kochi Univ. Of Technol.

• Uchida Hidenobu

  School Of Environmental Science And Engineering Kochi University Of Technology

• Yamashita Hirofumi

  Graduate School Of Human And Environmental Sciences Kyoto Prefectural University

• Anai Toyoaki

  Department of Applied Biological Sciences, Faculty of Agriculture, Saga University

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