Agrobacterium-Mediated Transformation of Euphorbia tirucalli Callus
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概要
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In order to establish a basis for transformation technology in the petroleum plant Euphorbia tirucalli, the callus of the plant was infected with Agrobacterium, washed with distilled water, sterilized with distilled water containing 100 mg/l of carbenicillin, selected on solidified B5 medium containing 13 mg/l of G418 and 100 mg/l of carbenicillin, and then on solidified B5 medium containing 25 mg/l of G418 and 100 mg/l of carbenicillin for the transgenic calli, and then the callus lines were subcultured successively on solidified B5 medium containing 50 mg/l of G418. We performed PCR analysis of sterilized G418-resistant callus line DNA and concluded that the gene introduced was integrated into the callus genome.
- 社団法人 日本農芸化学会の論文
著者
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UCHIDA Hidenobu
School of Environmental Science and Engineering, Kochi University of Technology
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YAMASHITA Hirofumi
Graduate School of Human and Environmental Sciences, Kyoto Prefectural University
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MURANAKA Toshiya
RIKEN Plant Science Center
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OHYAMA Kanji
Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University
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Uchida Hidenobu
School Of Environmental Sci. And Engineering Kochi Univ. Of Technol.
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Uchida Hidenobu
School Of Environmental Science And Engineering Kochi University Of Technology
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Yamashita Hirofumi
Graduate School Of Human And Environmental Sciences Kyoto Prefectural University
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Anai Toyoaki
Department of Applied Biological Sciences, Faculty of Agriculture, Saga University
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