Detection of 5-Lipoxygenase Activity in the Liverwort Marchantia polyrnorpha L.
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概要
- 論文の詳細を見る
We detected 5-LOX (arachidonate 5-lipoxygenase) in the homogenate of Marchantia polymorpha by spectrophotometry and mass spectrometry. LC/MS/MS analysis indicated that the liverwort 5-LOX produced 5-hydroperoxy-6,8,11,14-eicosatetraenoic acid (5-HPETE) with arachidonic acid as a substrate. The 5-LOX activity showed a Ca2+ response, as demonstrated for human 5-LOX. These findings suggest that the liverwort utilizes an arachidonate cascade in a defense signal response.
- 社団法人 日本農芸化学会の論文
- 2009-11-23
著者
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OHYAMA Kanji
Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University
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Ohyama Kanji
Res. Inst. For Bioresources And Biotechnology Ishikawa Prefectural Univ.
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KANAMOTO Hirosuke
Plant Research Group, Research Institute of Innovative Technology for the Earth (RITE)
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Ohyama Kanji
Plant Molecular Biology Laboratory Research Institute For Bioresources And Biotechnology Ishikawa Pr
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Takemura Miho
Plant Molecular Biology Laboratory Research Institute For Bioresources And Biotechnology Ishikawa Pr
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Kanamoto Hirosuke
Plant Molecular Biology Laboratory Research Institute For Bioresources And Biotechnology Ishikawa Pr
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Takemura Miho
Plant Molecular Biology Lab. Res. Inst. For Bioresources And Biotechnology Ishikawa Prefectural Univ
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