Detection of 5-Lipoxygenase Activity in the Liverwort Marchantia polymorpha L.

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概要

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• We detected 5-LOX (arachidonate 5-lipoxygenase) in the homogenate of Marchantia polymorpha by spectrophotometry and mass spectrometry. LC/MS/MS analysis indicated that the liverwort 5-LOX produced 5-hydroperoxy-6,8,11,14-eicosatetraenoic acid (5-HPETE) with arachidonic acid as a substrate. The 5-LOX activity showed a Ca2+ response, as demonstrated for human 5-LOX. These findings suggest that the liverwort utilizes an arachidonate cascade in a defense signal response.

著者

• KANAMOTO Hirosuke

  Plant Research Group, Research Institute of Innovative Technology for the Earth (RITE)

• TAKEMURA Miho

  Plant Molecular Biology Laboratory, Research Institute for Bioresources and Biotechnology, Ishikawa

• OHYAMA Kanji

  Plant Molecular Biology Laboratory, Research Institute for Bioresources and Biotechnology, Ishikawa

関連論文

• Detection of 5-Lipoxygenase Activity in the Liverwort Marchantia polymorpha L.
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• Detection of 5-Lipoxygenase Activity in the Liverwort Marchantia polyrnorpha L.

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