Expressed sequence tags from callus of Euphorbia tirucalli : A resource for genes involved in triterpenoid and sterol biosynthesis

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概要

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• We report generation of 9,301 expressed sequence tags (ESTs) derived from callus cells of Euphorbia tirucalli in search of candidate genes involved in the triterpenoid and sterol biosyntheses. After assembling 4,342 redundant ESTs into 1,252 clusters, a total of 6,211 non-redundant sequences were obtained. Database search revealed that 4,449 out of the 6,211 sequences shared significant similarities to known nucleotide or amino acid sequences, while the remaining 1,762 showed no significant matches and appear to represent novel genes in E. tirucalli. The annotations assigned to the hit database entries suggest that 48 of the unique sequences are involved in triterpenoid and sterol biosyntheses. Although functions of genes tagged by the 48 sequences are yet to be determined, the EST resource described here should contribute to identification of genes participating in the triterpenoid and sterol biosyntheses in E. tirucalli.

• 日本植物細胞分子生物学会の論文
• 2004-12-01

著者

• UCHIDA Hidenobu

  School of Environmental Science and Engineering, Kochi University of Technology

• OHYAMA Kanji

  Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University

• Ohyama Kiyoshi

  Plant Science Center Riken

• FUKUZAWA Hideya

  Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University

• Ohyama Kanji

  Department Of Chemistry And Materials Science Tokyo Institute Of Technology

• Ohyama Kanji

  Res. Inst. For Bioresources And Biotechnology Ishikawa Prefectural Univ.

• Ohyama K

  Kyoto Univ. Kyoto Jpn

• Ohyama Kanji

  Div. Appl. Life Sci. Grad. Schl. Agr. Kyoto Univ.

• Ohyama Kanji

  Div. Of Appl. Life Sci. Grad. Sch. Of Agric. Kyoto Univ.

• Ohyama Kanji

  Graduate School Of Biological Sciences Nara Institute Of Science And Technology:laboratory Of Plant

• Ohyama Kanji

  Research Institute For Bioresources And Biotechnology Ishikawa Prefectural University

• UCHIDA Hidenobu

  Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University

• Fukuzawa Hideya

  Div. Of Integrated Life Sci. Graduate School Of Biostudies Kyoto Univ.

• Fukuzawa H

  Lab. Of Plant Molecular Biology Graduate School Of Biostudies Kyoto Univ.

• Fukuzawa Hideya

  Div. Of Appl. Life Sci. Grad. Sch. Of Agric. Kyoto Univ.

• Fukuzawa Hideya

  Division Of Integrated Life Science Graduate School Of Biostudies Kyoto University

• Uchida Hidenobu

  School Of Environmental Sci. And Engineering Kochi Univ. Of Technol.

• Uchida H

  Research Institute For Bioresources And Biotechnology Ishikawa Prefectural University

• Yamato Katsuyuki

  Division Of Integrated Life Science Graduate School Of Biostudies Kyoto University

• Yamato Katsuyuki

  Lab. Of Plant Molecular Biology Graduate School Of Biostudies Kyoto Univ.

• Ohyama Kanji

  Laboratory Of Plant Molecular Biology Department Of Agricultural Chemistry Faculty Of Agriculture Ky

• KAJIKAWA Masataka

  Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University

• KOHZU Yoshito

  Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University

• SAKATA Ryoko

  Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University

• Sakata Ryoko

  Division Of Integrated Life Science Graduate School Of Biostudies Kyoto University

• Kohzu Yoshito

  Div. Of Integrated Life Sci. Graduate School Of Biostudies Kyoto Univ.

• Ohyama Kanji

  Dept.agric.chem. Fac.agric. Kyoto Univ.

• Kohzu Yoshito

  Division Of Integrated Life Science Graduate School Of Biostudies Kyoto University

• Uchida Hidenobu

  Research Institute For Bioresources And Biotechnology Ishikawa Prefectural University

• Yamato Katsuyuki

  Graduate School Of Biostudies Kyoto University

• Kajikawa Masataka

  Division Of Integrated Life Science Graduate School Of Biostudies Kyoto University

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