Structure of a Co-Transcribed Gene Cluster, ndhl-frxB^3-ndh6-ndh4L, Cloned from the Filamentous Cyanobacterium Plectonema boryanum
スポンサーリンク
概要
- 論文の詳細を見る
The frxB gene found in chloroplast genomes encodes a bacterial ferredoxin-like (Fd-like) FeS protein, whose function has not been established. Using an frxB gene probe from liverwort, we have isolated and determined the nucleotide sequence of a 4.6-kb fragment of DNA that contains the frxB gene from the filamentous cyanobacterium Plectonema boryanum. The frxB gene from Plectonema encodes a protein of 194 amino acids that shows 66% sequence identity to that encoded by liverwort chloroplasts. The sequence contains two clusters of cysteine residues (C-X-X-C-X-X-C-X-X-X-C-P), typical of bacterial ferredoxins that chelate two [4Fe-4S] clusters. Such sequences are found in highly conserved regions in the seven available sequences of frxB-related proteins. In addition, we identified three ORFs homologous to the chloroplast ndh1, ndh6 and ndh4L genes that encode the components of a putative NADH dehydrogenase. They show 60%, 50% and 65% amino acid sequence identity to the respective gene products from liverwort chloroplasts, but they show a lower degree of similarity to the mitochondrial homologues. The ndh1. frxB, ndh6 and ndh4L genes, occurring as single genomic copies, are tightly linked to one another, and they are cotranscribed as a 2.8-kb mRNA which was identified by Northern blot analysis with gene-specific probes. These four genes occur in the same order in the chloroplast genomes of liverwort, tobacco and rice as they do in Plectonema. Therefore, the organization of ndh1-frxB-ndh6-ndh4L may represent a case of clustering and co-transcription of functionally related genes in both cyanobacteria and chloroplasts. These findings lend a support to the hypothesis that cyanobacteria and chloroplasts contain an enzyme complex that is closely related to the mitochondrial complex I, although such a highly organized eazyme complex has not been identified to date in an extensive survey of cyanobacterial NAD(P)H dehydrogenases.
- 日本植物生理学会の論文
著者
-
Takahashi Yasuhiro
Department of Internal Medicine (Division of Cardiology, Hepatology, Geriatrics and Integrated Medic
-
Matsubara Hiroshi
Department of Respirology, Graduate School of Medicine, Chiba University
-
Ohyama Kanji
Department Of Chemistry And Materials Science Tokyo Institute Of Technology
-
Ohyama Kanji
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
-
Fujita Y
Division Of Enzymology Institute For Protein Research Osaka University
-
Fujita Y
Department Of Biology Faculty Of Science Osaka University:(present)institute For Protein Research Os
-
Fujita Yuichi
Department Of Biology Faculty Of Science Osaka University
-
Miyamoto H
Hitachi Ltd. Tokyo Jpn
-
Takahashi Yasuhiro
Department Of Biology Faculty Of Science Osaka University
-
Shonai Fumio
Department of Biology, Faculty of Science, Osaka University
-
Kohchi Takayuki
Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University
-
Matsubara Hiroshi
Department Of Dermatology Graduate School Of Medicine Chiba University
-
Matsubara H
Department Of Biochemistry Faculty Of Science Okayama University Of Science
-
Kohchi Takayuki
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
-
Matsubara H
Development Department Dainippon Screen Manufacturing Co. Ltd.
-
Takahashi Y
Okayama Univ. Okayama Jpn
-
Ohyama Kanji
Institute Of Bioresources And Biotechnology Ishikawa Prefectural University
-
Matsumoto H
Department Of Earth And Space Science Graduate School Of Science Osaka University
-
Shonai Fumio
Department Of Biology Faculty Of Science Osaka University:(present)institute For Fundamental Researc
-
Fujita Y
Fukui Prefectural Univ. Fukui Jpn
-
Takahashi Yasuhiro
Department Of Biological Sciences Graduate School Of Science Osaka University
-
Matsubara Hiroshi
Deparlment Of Internal Medicine School Of Medicine Kyoto University
関連論文
- Triterpenoid levels are reduced during Euphorbia tirucalli L. callus formation
- PE-440 The Incidence of Perioperative Complete Atrioventricular Block Associated with Percutaneous Transluminal Septal Myocardial Ablation and the Indication of Permanent Pacemaker(PE074,Cardiomyopathy/Hypertrophy (Clinical) 2 (M),Poster Session (English)
- MpFAE3, aβ-Ketoacyl-CoA Synthase Gene in the Liverwort Marchantia polymorpha L., Is Preferentially Involved in Elongation of Palmitic Acid to Stearic Acid(Biochemistry & Molecular Biology)
- Functional Analysis of a β-Ketoacyl-CoA Synthase Gene, MpFAE2, by Gene Silencing in the Liverwort Marchantia polymorpha L.(Biochemistry & Molecular Biology)
- Multidetector-Row Computed Tomography Management of Acute Pulmonary Embolism
- Chemical Phenotypes of the hmg1 and hmg2 Mutants of Arabidopsis Demonstrate the In-planta Role of HMG-CoA Reductase in Triterpene Biosynthesis
- Increasing the Copy Number of rbcL Genes in Chlamydomonas reinhardtii Chloroplast Genome Affects neither Cell Proliferation nor Distribution of Pyrenoid Antigens to Rubisco Holoenzyme Antibody
- P-392 BIOSYNTHETIC PATHWAY OF PHYTOSTEROLS VIA LANOSTEROL
- Synthesis of Rubisco gene products is upregulated by increasing the copy number of rbcL gene in Chlamydomonas chloroplast genome, without increased accumulation of the two Rubisco subunits
- Ring/marker chromosome derived from chromosome 7 in childhood acute megakaryoblastic leukemia with monosomy 7