The Isolation and Characterization of a Cytochrome b_6f Complex from the Cyanobacterium Spirulina sp.

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概要

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• A cytochrome b_6f complex was isolated and purified from Spirulina sp. The complex was solubilized with n-heptyl β-D-thioglucoside and chromatographed on a DEAE-Toyopearl 650M column. The purified complex contained a small amount of chlorophyll and carotenoid. At least four polypeptides were present in the complex: cytochrome f (29 kDa), cytochrome b_6 (23 kDa), iron-sulfur protein (ISP, 23 kDa), and a 17 kDa polypeptide. Each polypeptide was separated from the complex treated with 2-mercaptoethanol or urea. The absorption spectra of cytochrome b_6 and cytochrome f were similar to those of Anabaena and spinach as expected. The complex was active in supporting ubiquinol-cytochrome c oxidoreductase activity. Fifty percent inhibition of the activity was accomplished by 1 μM dibromothymoquinone (DBMIB). The K_m values for ubiquinol-2 and cytochrome c (horse heart) were 5.7 μM and 7.4 μM, respectively.

• 日本植物生理学会の論文

著者

• Wada Keishiro

  Department Of Biology Faculty Of Science Osaka University

• Wada K

  Department Of Biology Faculty Of Sciences Kanazawa University

• Wada Keishiro

  Department Of Bioiogy Faculty Of Science Kanazawa University

• Matsubara Hiroshi

  Department of Respirology, Graduate School of Medicine, Chiba University

• MINAMI Yoshiko

  Department of Biochemistry, Faculty of Science, Okayama University of Science

• Minami Yoshiko

  Department Of Biology Faculty Of Sciences Osaka University

• Minami Yoshiko

  Department Of Biochemistry Faculty Of Science Okayama University Of Science

• Matsubara Hiroshi

  Department Of Biology Faculty Of Sciences Osaka University

• Matsubara Hiroshi

  Deparlment Of Internal Medicine School Of Medicine Kyoto University

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